Cigarette smoke extract triggers neoplastic change in lungs and impairs locomotor activity through wnt3a- β-catenin signaling in aged COPD rodent model.
Cigarette smoke extract triggers neoplastic change in lungs and impairs locomotor activity through wnt3a-β-catenin signaling in aged COPD rodent model. Exp Lung Res. 2020 Jul 30;:1-14 Authors: Devi K, Moharana B Abstract BACKGROUND: Chronic cigarette smoking primes immense decline in lung functions and retardation of motor functions with increase in age. This raise the question of whether age status overwhelm the susceptibility to smoking induced lung inflammatory diseases and neuro-motor dysfunctions. METHODS: To study the hypothesis 11-12 month old aged wistar rats (n = ...
Source: Experimental Lung Research - July 31, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Severe, transient pulmonary ventilation-perfusion mismatch in the lung after porcine high velocity projectile behind armor blunt trauma.
Authors: Rocksén D, Arborelius UP, Gustavsson J, Günther M Abstract BACKGROUND: Behind armor blunt trauma (BABT) is a non-penetrating injury caused by the rapid deformation of body armor, by a projectile, which may in extreme circumstances cause death. Although there is not a high incidence of high energy BABT, the understanding of the mechanisms is still low, in relation to what is needed for safety threshold levels. BABT is also useful as a model for blunt thoracic trauma, with a compressive speed between traffic accidents and blast caused by explosives. High velocity projectile BABT causes severe hyp...
Source: Experimental Lung Research - July 24, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

First-time handling of different inhalers by chronic obstructive lung disease patients.
Authors: Harb HS, Laz NI, Rabea H, Abdelrahim MEA Abstract Background: There is a lack of guidance on inhaler device selection and how to individualize inhaler choice when prescribed for the first-time.Aim of the work: To compare different inhalers regarding ease of use and number of counseling attempts needed for correct handling in subjects with a first experience to such inhalers; also, to investigate if there is a correlation between total correct steps achievements and patient demographics/clinical variables.Method: An open-label, non-drug interventional, cross-over study was conducted including 180 Egyptian p...
Source: Experimental Lung Research - July 4, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Blocking protein phosphatase 2A with a peptide protects mice against bleomycin-induced pulmonary fibrosis.
Authors: Yu J, Deng Y, Han M Abstract Emerging data indicate that endothelial-mesenchymal transition (EndMT) is involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). A previous study noted that blocking the activity of protein phosphatase 2 A (PP2A) could attenuate EndMT. However, the treatment effects of PP2A inhibitors in pulmonary fibrosis remain not investigated. In the present study, we used a PP2A inhibitor, a newly designed peptide named TAT-Y127WT, to determine the role of PP2A in pulmonary fibrosis. Herein, we showed that TAT-Y127WT protected mice against BLM-induced pulmonary fibrosi...
Source: Experimental Lung Research - June 27, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Pulmonary delivery of ORMDL3 short hairpin RNA - a potential tool to regulate allergen-induced airway inflammation.
Conclusions: Post-transcriptional down regulation of ORMDL3 in allergic lungs using i.n-delivered ORMDL3 shRNA (akin to inhaled therapy) attenuates development of key features of airway allergic disease, confirming the involvement of ORMDL3 in allergic asthma pathogenesis and serving as a model for a potential therapeutic strategy. PMID: 32578458 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - June 26, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.
Conclusions: Our results demonstrated that p53 upregulated GPRC5a expression in NSCLC cells, and the loss of p53 expression in NSCLC may be one of the mechanisms leading to the decreased GPRC5a expression in NSCLC. PMID: 32410473 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - May 17, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Post-treatment with a heat shock protein 90 inhibitor prevents chronic lung injury and pulmonary fibrosis, following acute exposure of mice to HCl.
Conclusions: We conclude that AUY-922, and possibly other HSP90 inhibitors, successfully block the adverse effects associated with acute exposures to HCl and may represent an effective antidote against HCl-induced chronic lung injury and fibrosis. PMID: 32400213 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - May 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Influence of silica particles on mucociliary structure and MUC5B expression in airways of C57BL/6 mice.
This study aims to evaluate the effects of silica particles on mucociliary structure and MUC5B production in respiratory tract.Materials and Methods: C57BL/6 mice were administered with 2.5 mg silica particles through a single intratracheal instillation. The changes of mucociliary structure and MUC5B expression in trachea was evaluated by HE and AB-PAS staining, transmission electron microscopy and immunohistochemistry on days 1, 7, 28 and 84 post-exposure.Results: The mucociliary structure of airway epithelium was obviously impaired by silica particles, showing disordered, shortened or partially lost cilia on the s...
Source: Experimental Lung Research - May 8, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

LincRNA-p21 promotes classical macrophage activation in acute respiratory distress syndrome by activating NF- κB.
Conclusion: This study revealed elevated lincRNA-p21 levels in LPS-induced ARDS and investigated the potential role of lincRNA-p21 in LPS-induced pro-inflammatory response via NF-κB/p65 mediated pathways, suggesting the potential application of lincRNA-p21 for ADRS therapy. PMID: 32362153 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - May 5, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Schisandrin B attenuates bleomycin-induced pulmonary fibrosis in mice through the wingless/integrase-1 signaling pathway.
Conclusions: The present study provided evidence that Sch B may be a potential therapeutic agent for the treatment of PF. PMID: 32362157 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - May 5, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

ISG56 is involved in CXCL10 expression induced by TLR3 signaling in BEAS-2B bronchial epithelial cells.
Conclusions: ISG56 was induced by poly IC through TLR3/IFN-β axis, and ISG56 may positively regulated CXCL10 expression in BEAS-2B cells. ISG56 may modulate anti-viral innate immunity, at least in part, by regulating the expression of CXCL10 in bronchial epithelial cells. PMID: 32363951 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - May 5, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

The roles of dipeptidyl peptidase-4 and its inhibitors in the regulation of airway epithelial-mesenchymal transition.
Conclusion: The TGF-β1-induced EMT of human bronchial epithelial cells could be promoted by sCD26/sDPP4. The suppression of EMT in human bronchial epithelial cells was achieved by DPP4 inhibitor, and the TGF-β1-mediated EMT of human airway cells was promoted by the synergy of IL-17 and sCD26/sDPP4 in vitro. PMID: 32292085 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - April 17, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Stromal cell-derived factor-1 (SDF-1) expression in very preterm human lungs: potential relevance for stem cell therapy for bronchopulmonary dysplasia.
Authors: De Paepe ME, Wong T, Chu S, Mao Q Abstract Background: The axis formed by CXC chemokine receptor 4 (CXCR4), expressed on mesenchymal stromal cells (MSCs), and stromal cell-derived factor-1 (SDF-1), expressed in recipient organs, is a critical mediator of MSC migration in non-pulmonary injury models. The role and regulation of SDF-1 expression in preterm lungs, of potential relevance for MSC-based cell therapy for bronchopulmonary dysplasia (BPD), is unknown. The aim of this study was to determine the spatiotemporal pattern of CXCR4/SDF-1 expression in lungs of extremely preterm infants at risk for BPD.Meth...
Source: Experimental Lung Research - April 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Dapsone as treatment adjunct in ARDS.
Authors: Kast RE Abstract Multiple pharmacological interventions tested over the last decades have failed to reduce ARDS mortality. This short note recounts past data indicating that (i) neutrophils home along an IL-8 gradient, (ii) in ARDS, massive neutrophil accumulation and degranulation in and along bronchoalveolar spaces contributes to damage and hypoxia, (iii) large increases in IL-8 are one of the chemotaxic signals drawing neutrophils to the ARDS lung, and (iv) old data from dermatology and glioblastoma research showed that the old drug against Hansen's disease, dapsone, inhibits neutrophils' chemotaxis to ...
Source: Experimental Lung Research - April 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Feasibility of pleural and perilesional subcutaneous microdialysis to assess porcine experimental pulmonary contusion.
Authors: Günther M, Sondén A, Gustavsson J, Arborelius UP, Rocksén D Abstract Background: Severe thoracic trauma affects 55% of patients with multiple traumatic injuries and may lead to acute lung injury or acute respiratory distress syndrome. Pulmonary trauma differs clinically and biologically from lung injury of other origins and carries a mortality rate of 10%. Treatment options are limited, and it is not possible to monitor the progression of lung injury with specific biomarkers. Microdialysis of pleural fluid may offer a viable entry to monitor the lung directly and specifically. Bronchial ...
Source: Experimental Lung Research - March 19, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Anti-oxidative effect of the tyrosine kinase inhibitor nintedanib: a potential therapy for chronic lung allograft dysfunction?
Conclusion: These results demonstrate a downregulation of ROS and TGM-2 by pretreatment with the receptor tyrosine kinase inhibitor nintedanib and present its potential anti-oxidative and immunomodulatory effect in the treatment of chronic lung allograft dysfunction. PMID: 32169022 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - March 16, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

The expression of β-Defensin-2, IL-22, IL-22R1 and IL-10R2 in rat model of Klebsiella pneumonia and their correlation with histological grades.
The expression of β-Defensin-2, IL-22, IL-22R1 and IL-10R2 in rat model of Klebsiella pneumonia and their correlation with histological grades. Exp Lung Res. 2020 Mar 13;:1-8 Authors: Fan J, Luo Y, Qin Y, Wu C, Han X, Ouyang H, Zhang L, Cai P, Li N Abstract Backgrounds and Aims: Klebsiella pneumoniae represents the most common opportunistic pathogen contributing to Klebsiella pneumonia in hospital-acquired infections. Klebsiella pneumonia has a rapidly progressive clinical course and multi-drug resistant (MDR). Identification of the effective biochemical markers is crucial for improving early diag...
Source: Experimental Lung Research - March 16, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

IL-27 alleviates airway remodeling in a mouse model of asthma via PI3K/Akt pathway.
Conclusions: IL-27 can alleviate airway remodeling in OVA-induced asthmatic mice, and the mechanism may relate to PI3K/Akt pathway. PMID: 32164467 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - March 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Innate inflammatory response to acute inhalation exposure of riot control agent oleoresin capsicum in female rats: An interplay between neutrophil mobilization and inflammatory markers.
Conclusion: Results of the present study demonstrated that, exposure to OC spray may mitigate inflammatory response and development of acute lung injury in rats. However, it can be concluded that although OC spray causes pulmonary hazards in the aforementioned concentrations, it can be used as a non-lethal riot control agent in minimal concentration. Understanding the in-depth mechanism of action in the molecular and receptor level will help in developing effective antagonist against OC. PMID: 32131645 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - March 6, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

TGF- β1 increases sialidase 3 expression in human lung epithelial cells by decreasing its degradation and upregulating its translation.
Conclusion: TGF-β1 upregulates levels of NEU3 in epithelial cells by both decreasing NEU3 degradation and by increasing the translation of NEU3 mRNA, explaining the apparent paradox of high levels of NEU3 protein in pulmonary fibrosis without a concomitant increase in the expression of NEU3 mRNA. PMID: 32102576 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - February 28, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Effects of long term normobaric hyperoxia exposure on lipopolysaccharide-induced lung injury.
Conclusions: Exposure to hyperoxia did not augment lung injury in the LPS-induced lung injury model, and some indicators even showed better outcomes. These results suggest that long-term high-oxygen therapy in patients with SLI has low risk of lung injury. PMID: 32067505 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - February 20, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Aluminum hydroxide nebulization-induced redox imbalance and acute lung inflammation in mice.
Conclusion: In conclusion, short-term nebulization with aluminum hydroxide induces the influx of inflammatory cells and oxidative stress in adult BALB/c mice. PMID: 32067522 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - February 20, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Thymoquinone treatment modulates the Nrf2/HO-1 signaling pathway and abrogates the inflammatory response in an animal model of lung fibrosis.
Authors: Ahmad A, Alkharfy KM, Jan BL, Ahad A, Ansari MA, Al-Jenoobi FI, Raish M Abstract The present study investigates the therapeutic potential of thymoquinone (TQ) in bleomycin-induced lung fibrosis (BMILF) and elucidates the target-signaling pathway for its effect. Lung fibrosis was induced in rats by a single intra-tracheal instillation of bleomycin (BM) (6.5 U/kg) followed by thymoquinone treatment (10 and 20 mg/kg p.o.) for 28 days. Control rats received saline instead of TQ. Changes in body weight, inflammatory cells count, cytokines levels, and biochemical parameters of the broncho-al...
Source: Experimental Lung Research - February 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Deep inspirations attenuate postprandial airway inflammation in college-aged adults with elevated baseline exhaled nitric oxide: A pilot study.
Authors: Kurti SP, Smith JR, Rosenkranz SK, Emerson SR, Edwards ES, Jurrens K, Laughlin A, Harms CA Abstract Airway inflammation (assessed by exhaled nitric oxide (eNO)) increases after a single high-fat meal (HFM), yet this response may be modified by airway stretch and baseline eNO level.Purpose: The purpose of this study was to investigate whether deep inspirations (DIs) would attenuate airway inflammation post-HFM and whether this is modulated by baseline eNO level.Methods: A total of sixteen healthy college-aged participants completed a randomized cross-over study with 8 lower eNO (14.8 ± ...
Source: Experimental Lung Research - January 18, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Dioscin alleviates lipopolysaccharide-induced acute lung injury through suppression of TLR4 signaling pathways.
Conclusions:In brief, the results indicated that dioscin alleviates LPS-induced ALI through suppression of TLR4 signaling pathways. PMID: 31931639 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - January 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Methacholine dose response curve and acceptability criteria of respiratory mechanics modeling.
This study aimed to analyze the Constant Phase Model (CPM) Coefficient of Determination (COD) and an index of harmonic distortion ([Formula: see text]) behavior in intravenous methacholine dose response curve. We studied the COD and [Formula: see text] behavior of Control and Lung Inflammation (OVA) groups of mice and we proposed an alternative for moments when the CPM should not be applied. Methods: 9-week female BALB/c mice were studied, 8 of the control group (23.11 ± 1.27 g) and 11 of the lung inflammation group (OVA) (21.45 ± 2.16 g). The COD values were obtained...
Source: Experimental Lung Research - January 15, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

14-3-3 β Is necessary in the regulation of polarization and directional migration of alveolar myofibroblasts by lipopolysaccharide.
Conclusions:Taken together, our findings suggest that LPS exposure may increase RhoA activity of myofibroblasts by TGF-α/EGFR/14-3-3β signaling pathway, and then disturb myofibroblasts polarization and directional migration. PMID: 31920140 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - January 12, 2020 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

RAGE and AXL expression following secondhand smoke (SHS) exposure in mice.
Conclusions: Our results suggest that there is a possible correlation between RAGE and AXL during SHS exposure. Additional research is critically needed that dissects the molecular interplay between these two important signaling cascades. At this point, the current studies provide insight into tobacco-mediated effects in the lung and clarify possible avenues for alleviating complications that could arise during SHS exposure such as those observed during COPD exacerbations. PMID: 31762322 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - November 26, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Bioinformatics analysis on differentially expressed genes of alveolar macrophage in IPF.
Conclusions: The up-regulation of genes such as SPP1 and PPBP affect the secretion of alveolar macrophages, thereby speeding up the process of fibrosis. PMID: 31762326 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - November 26, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

The efficacy of pirfenidone in a sheep model of pulmonary fibrosis.
This study showed that pirfenidone can attenuate bleomycin-induced pulmonary fibrosis in sheep, and can therefore be used as a positive control to assess other novel therapeutics for IPF in this model. PMID: 31762329 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - November 26, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Pravastatin alleviates allergic airway inflammation in obesity-related asthma mouse model.
Conclusions: Pravastatin treatment in obese asthmatic mice suppressed allergic airway infiltration and AHR by inhibition of Th2 and Th17-associated signaling pathways, decreasing the leptin expression and downstream p38 MAPK signaling pathways. The effect on lean asthmatic mice was different, independent of airway cell counts and cytokines. PMID: 31608695 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - October 16, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Techniques for lung transplantation in the rat.
Authors: Rajab TK Abstract The rat is an important model organism for lung transplantation research. Orthotopic rat lung transplantation is a complex procedure, which requires advanced microsurgical techniques. This technical paper describes in detail highly reproducible intraoperative microsurgical techniques and peri-operative management, including the donor procedure, recipient anesthetic management, the recipient operation and anticipated peri-operative complications. PMID: 31608698 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - October 16, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Pseudomonas aeruginosa-derived flagellin stimulates IL-6 and IL-8 production in human bronchial epithelial cells: A potential mechanism for progression and exacerbation of COPD.
Conclusions: P. aeruginosa-derived flagellin-induced IL-6 and IL-8 production in bronchial epithelial cells, which partially explains the mechanisms of progression and exacerbation of COPD. Corticosteroids are the most effective treatment for the suppression of flagellin-induced IL-6 and IL-8 production in the bronchial epithelial cells. PMID: 31517562 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - September 14, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine model of allergic asthma.
Conclusions: In a murine model of allergic asthma, ANP could inhibit TGF-β1-induced EMT of bronchial epithelial cells through cGMP/PKG signaling, targeting TGF-β1/Smad3 via attenuating phosphorylation of Smad3 in vitro, which may provide potential of ANP in treating allergic asthma with airway remodeling. PMID: 31496319 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - September 10, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Dioscin attenuates Bleomycin-Induced acute lung injury via inhibiting the inflammatory response in mice.
Conclusion: Our data indicate that dioscin attenuates oxidative stress, the lung inflammatory response, and acute lung injury in BLM-challenged mice. PMID: 31452411 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - August 28, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Thymic stromal lymphopoietin induced early stage of epithelial-mesenchymal transition in human bronchial epithelial cells through upregulation of transforming growth factor beta 1.
Conclusions: TSLP could induce early stage of EMT in airway epithelial cells through upregulation of TGF-β1. This effect may act as a targeting point for suppression of asthma. PMID: 31378088 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - August 7, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Alpha 1-antitrypsin for treating ventilator-associated lung injury in acute respiratory distress syndrome rats.
Conclusions: AAT ameliorated VILI in ARDS rats. The protection conferred by AAT may be associated with the anti-inflammatory, antioxidative stress response and anti-apoptotic effects of AAT. PMID: 31347410 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 28, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Restrictive lung disease in TNF-transgenic mice: correlation of pulmonary function testing and micro-CT imaging.
Conclusions: These findings demonstrate that: 1) TNF-Tg mice display a restrictive pathology, and 2) in vivo µCT is a valid outcome measure to infer changes in pulmonary mechanical and functional parameters. PMID: 31318607 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 19, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

The effect of levofloxacin on the lung microbiota of laboratory rats.
Authors: Finn SMB, Scheuermann U, Holzknecht ZE, Gao Q, Ibrahim MM, Parker W, Granek JA, Lin SS, McKenney EA, Barbas AS Abstract Aim: The aim of this study was to investigate the short-term effect of levofloxacin on the microbiota of healthy lungs. Material and methods: Male F344 rats received either no levofloxacin (n = 9), intravenous levofloxacin (n = 12), oral levofloxacin (n = 12), or subcutaneous levofloxacin (n = 14). Rats received a clinically applicable dose (5.56 mg/kg) of levofloxacin via the assigned delivery route once daily for three days....
Source: Experimental Lung Research - July 14, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

The novel inhibitor PRI-724 for Wnt/ β-catenin/CBP signaling ameliorates bleomycin-induced pulmonary fibrosis in mice.
Conclusions: These results suggest that the β-catenin/CBP inhibitor PRI-724 is a potent antifibrotic agent that acts by modulating the activity of macrophages in the lungs. PMID: 31298961 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 14, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects.
Conclusion: These data provide evidence that unphysiological mechanical stretching of lung cells induced several profibrotic effects and accelerated EMT, which may have critical implications in terms of development or aggravation of pulmonary fibrosis in the clinical context. PMID: 31290711 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 12, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Schisandrin B inhibits TGF- β1-induced epithelial-mesenchymal transition in human A549 cells through epigenetic silencing of ZEB1.
Conclusions: Our data suggest that Sch B can prevent TGF-β1-stimulated EMT in A549 cells through epigenetic silencing of ZEB1, which may be clinically related to the efficient treatment of EMT-associated fibrotic diseases. PMID: 31268360 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 5, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Isolation and culture of pleural mesothelial cells.
Conclusions: This is a simple procedure for the efficient collection of primary pleural mesothelial cells, which were obtained in defined culture conditions from the euthanized rodent thoracic cavity using trypsin-EDTA treatment. The ability to easily culture and maintain identifiable pleural mesothelial cells from rodents will be helpful for future experiments using these cells. PMID: 31250673 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - July 1, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Peptide PD29 treats bleomycin-induced pulmonary fibrosis by inhibiting the TGF- β/smad signaling pathway.
In this study, fibrotic lung injuries were induced in SD rats by a single intratracheal instillation of 5 mg/kg bleomycin (BLM). Then, these rats were administered 7.5, 5, or 2.5 mg/kg PD29 daily for 30 days. BLM induced-syndromes including structure distortion, excessive deposition of ECM, excessive inflammatory infiltration, and pro-inflammatory cytokine release were used to evaluate the protective effect of PD-29. Oxidative stress damage in lung tissues was attenuated by PD29 in a dose-dependent manner. The expression of TGF-β1 and the phosphorylation of Smad-2/-3-its downstream targets-were en...
Source: Experimental Lung Research - June 19, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Neutrophil mediated inflammatory lung damage following single Sub lethal inhalation exposure to plant protein toxin abrin in mice.
Authors: Sant B, Kumar P, Soni AK, Kannan GM, Nagar DP, Prasad GBKS, Bhaskar ASB Abstract Abrin, a highly toxic plant protein found in the seeds of Abrus precatorius plant. To date, there is no antidote against abrin intoxication. Abrin is toxic by all routes of exposure, but inhalation exposure is the most toxic of all routes. Present study was conducted to evaluate the acute inhalation toxicity of aerosolized abrin in BALB/c mice. Animals were exposed to 0.2 and 0.8LC50 doses of aerosolized abrin and evaluated at 1 and 3 day post toxin exposure. Bronchoalveolar fluid from lungs was used for evaluation of m...
Source: Experimental Lung Research - June 15, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

The effects of aging and exercise on lung mechanics, surfactant and alveolar macrophages.
Conclusions: Our data supports previous literature that suggest the development of emphysema-like alterations to lung mechanics with aging. This effect was independent of exercise. Our data also indicates that surfactant is unaffected by aging and exercise. Alveolar macrophage properties and numbers were affected by exercise in the aging lung and may represent the main, potentially beneficial, effect of exercise on the pulmonary system. PMID: 31195852 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - June 15, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Hypermethylation of mitochondrial transcription factor A induced by cigarette smoke is associated with chronic obstructive pulmonary disease.
Conclusions: Cigarette smoke-induced hypermethylation of the mtTFA promoter is related to the initiation and progression of COPD. Our finding may provide a new strategy for the intervention of COPD by developing demethylation agents targeting mtTFA hypermethylation. PMID: 31198067 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - June 15, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Beryllium inhibits apoptosis via mitochondria in beryllium-induced lung disease in the rat.
Conclusions: We conclude that inhibition of apoptosis by beryllium oxide involves mitochondrial apoptosis pathway in rat model of beryllium oxide-induced pulmonary disease. PMID: 31155972 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - June 5, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Inhaled salbutamol from aerolizer and diskus at different inhalation flows, inhalation volume and number of inhalations in both healthy subjects and COPD patients.
Authors: Boshra MS, Almeldien AG, Eldin RS, Elberry AA, Abdelwahab NS, Salem MN, Rabea H, Abdelrahim MEA Abstract The aim of the present study was to demonstrate the effect of inhalation-flow, inhalation-volume and number of inhalations on aerosol-delivery of inhaled-salbutamol from two different dry powder inhalers (DPIs) in both healthy-subjects and chronic obstructive pulmonary disease (COPD) patients. Relative pulmonary-bioavailability and systemic-bioavailability of inhaled-salbutamol, delivered by Diskus and Aerolizer, was determined in 24-COPD patients and 24-healthy subjects. The healthy-subjects and the CO...
Source: Experimental Lung Research - June 5, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

FOXP3 infiltrating lymphocyte density and PD-L1 expression in operable non-small cell lung carcinoma.
Conclusion: Tumor stroma infiltration by FOXP3+ Tregs is an early event in the progression of NSCLC. Low lymphocytic infiltration defines poor prognosis, which becomes worse when the small numbers of infiltrating lymphocytes characterizing these tumors contain FOXP3 + Tregs. Furthermore, the direct association of FOXP3+ Treg infiltration density with PD-L1 expression by cancer cells implies a co-ordinated immune-suppressive activity in NSCLC. PMID: 31134811 [PubMed - as supplied by publisher] (Source: Experimental Lung Research)
Source: Experimental Lung Research - May 29, 2019 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research