The < b > < i > PMEL < /i > < /b > Gene and Merle in the Domestic Dog: A Continuum of Insertion Lengths Leads to a Spectrum of Coat Color Variations in Australian Shepherds and Related Breeds
Merle is a distinct coat color and pattern found in numerous species, including the domestic dog, characterized by patches of diluted eumelanin (black pigment) interspersed among areas of normal pigmentation. In dogs, this variegated pattern is caused by an insertion of a SINE element into the caninePMEL gene. Although variation in the length of the SINE insertion - due to a variable-length poly(A) tail - has been observed to be associated with variation in merle coat color and patterning, no systematic evaluation of this correlation has been conducted and published in the scientific literature. We performed high-resolutio...
Source: Cytogenetic and Genome Research - August 2, 2018 Category: Genetics & Stem Cells Source Type: research
siRNA Targeting of the < b > < i > SNCG < /i > < /b > Gene Inhibits the Growth of Gastric Carcinoma SGC7901 Cells in vitro and in vivo < b > < /b > by Downregulating the Phosphorylation of AKT/ERK
The aim of the study was to evaluate the effects of synuclein-#x03B3; (SNCG) silencing on gastric cancer SGC7901 cells and to elucidate the associated mechanisms. pGCSIL-lentiviral siRNA targeting of theSNCG gene was employed to inhibitSNCG expression. Several experiments such as quantitative real-time PCR, Western blotting, MTT, colony formation, migration assay, and flow cytometry were performed to investigate the biological behavior of infected SGC7901 cells. BALB/c nude mice were used as tumor xenograft models to assess the effects ofSNCG silencing on tumor growth. Western blot analysis was carried out to determine the...
Source: Cytogenetic and Genome Research - July 20, 2018 Category: Genetics & Stem Cells Source Type: research
Comparative Cytogenetics of Omophoita abbreviata and O. aequinoctialis (Coleoptera, Chrysomelidae, Alticini) from the Adolpho Ducke Forest Reserve in Brazilian Amazonia: Intrapopulation Variation in Karyotypes
The chromosomes of 2 flea beetle species from central Amazonia,Omophoita abbreviata andO. aequinoctialis (Alticini), were investigated through analysis of meiotic and mitotic cells. These species belong to the subtribe Oedionychina, a taxon that has unique cytogenetic features, such as giant sex chromosomes which are aligned at a distance during meiosis I (asynaptic).O. abbreviata andO. aequinoctialishave a meiotic formula of 10II + X + y, which is predominant in this subtribe. While the species of the genusOmophoita possess a relatively stable karyotype, a typical feature for Oedionychina, the present study identified int...
Source: Cytogenetic and Genome Research - July 19, 2018 Category: Genetics & Stem Cells Source Type: research
Angelman Syndrome-Affected Individual with a Numerically Normal Karyotype and Isodisomic Paternal Uniparental Disomy of Chromosome 15 due to Maternal Robertsonian Translocation (14;15) by Monosomy Rescue
Angelman syndrome (AS) is a neurodevelopmental disorder caused by deletion of the maternally inherited 15q11q13 region, paternal uniparental disomy 15 [upd(15)pat], an imprinting defect of the maternal chromosome region 15q11q13, or a pathogenic mutation of the maternalUBE3Aallele. Predisposing factors for upd(15)pat, such as nonhomologous robertsonian translocation involving chromosome 15, have been discussed, but no evidence for this predisposition has been published. In the present study, chromosomal analysis was performed in a child with AS, both parents, and the maternal grandparents. Methylation-specific multiplex li...
Source: Cytogenetic and Genome Research - July 17, 2018 Category: Genetics & Stem Cells Source Type: research
Contrasting Rates of LINE-1 Amplification among New World Primates of the Atelidae Family
LINE-1 (L1) retrotransposons constitute the dominant category of transposons in mammalian genomes. L1 elements are active in the vast majority of mammals, and only a few cases of L1 extinction have been documented. The only possible case of extinction in primates was suggested for South American spider monkeys. However, these previous studies were based on a single species. We revisited this question with a larger phylogenetic sample, covering all 4 genera of Atelidae and 3 species of spider monkeys. We used an enrichment method to clone recently inserted L1 elements and performed an evolutionary analysis of the sequences....
Source: Cytogenetic and Genome Research - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
