siRNA Targeting of the < b > < i > SNCG < /i > < /b > Gene Inhibits the Growth of Gastric Carcinoma SGC7901 Cells in vitro and in vivo < b > < /b > by Downregulating the Phosphorylation of AKT/ERK

The aim of the study was to evaluate the effects of synuclein-#x03B3; (SNCG) silencing on gastric cancer SGC7901 cells and to elucidate the associated mechanisms. pGCSIL-lentiviral siRNA targeting of theSNCG gene was employed to inhibitSNCG expression. Several experiments such as quantitative real-time PCR, Western blotting, MTT, colony formation, migration assay, and flow cytometry were performed to investigate the biological behavior of infected SGC7901 cells. BALB/c nude mice were used as tumor xenograft models to assess the effects ofSNCG silencing on tumor growth. Western blot analysis was carried out to determine the relative levels of AKT, p-AKT, ERK, and p-ERK expression. Our results showed thatSNCGwas overexpressed in SGC7901 cells as compared to normal gastric mucosal epithelial cells. SGC7901 cells transfected withSNCG siRNA demonstrated significantly decreased gastric cancer growth (p
Source: Cytogenetic and Genome Research - Category: Genetics & Stem Cells Source Type: research