Current Opinion in Structural Biology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Recent advances in data collection for Cryo-EM methods
Curr Opin Struct Biol. 2024 Mar 13;86:102795. doi: 10.1016/j.sbi.2024.102795. Online ahead of print.ABSTRACTMethods of transmission electron microscopy (TEM) are typically used to resolve structures of vitrified biological specimens using both single particle analysis (SPA) and tomographic methods and use both conventional as well as scanning transmission modes of data collection. Automation of data collection for each method has been developed to different levels of convenience for the users. Automation of methods using the conventional TEM mode has progressed the furthest. Beam-image shift strategies first used in data c...
Source: Current Opinion in Structural Biology - March 14, 2024 Category: Biology Authors: Anchi Cheng Yue Yu Source Type: research

Dynamics, allostery, and stabilities of whole virus particles by amide hydrogen/deuterium exchange mass spectrometry (HDXMS)
Curr Opin Struct Biol. 2024 Mar 7;86:102787. doi: 10.1016/j.sbi.2024.102787. Online ahead of print.ABSTRACTX-ray crystallography and cryo-electron microscopy have enabled the determination of structures of numerous viruses at high resolution and have greatly advanced the field of structural virology. These structures represent only a subset of snapshot end-state conformations, without describing all conformational transitions that virus particles undergo. Allostery plays a critical role in relaying the effects of varied perturbations both on the surface through environmental changes and protein (receptor/antibody) interact...
Source: Current Opinion in Structural Biology - March 8, 2024 Category: Biology Authors: Varun Venkatakrishnan Sean M Braet Ganesh S Anand Source Type: research

A comprehensive survey on protein-ligand binding site prediction
Curr Opin Struct Biol. 2024 Mar 5;86:102793. doi: 10.1016/j.sbi.2024.102793. Online ahead of print.ABSTRACTProtein-ligand binding site prediction is critical for protein function annotation and drug discovery. Biological experiments are time-consuming and require significant equipment, materials, and labor resources. Developing accurate and efficient computational methods for protein-ligand interaction prediction is essential. Here, we summarize the key challenges associated with ligand binding site (LBS) prediction and introduce recently published methods from their input features, computational algorithms, and ligand typ...
Source: Current Opinion in Structural Biology - March 6, 2024 Category: Biology Authors: Ying Xia Xiaoyong Pan Hong-Bin Shen Source Type: research

Navigating the complexities of multi-domain protein folding
Curr Opin Struct Biol. 2024 Mar 2;86:102790. doi: 10.1016/j.sbi.2024.102790. Online ahead of print.ABSTRACTProteome complexity has expanded tremendously over evolutionary time, enabling biological diversification. Much of this complexity is achieved by combining a limited set of structural units into long polypeptides. This widely used evolutionary strategy poses challenges for folding of the resulting multi-domain proteins. As a consequence, their folding differs from that of small single-domain proteins, which generally fold quickly and reversibly. Co-translational processes and chaperone interactions are important aspec...
Source: Current Opinion in Structural Biology - March 3, 2024 Category: Biology Authors: Nandakumar Rajasekaran Christian M Kaiser Source Type: research