Supplementation of extender with chamuangone-enriched Garcinia cowa leaf extract improves quality parameters and longevity of cold-stored cat semen
CONCLUSION: 100 µg/mL chamuangone can improve sperm characteristics during 15 days of preservation at 4 degree C, keeping sperm alive (49.3 ± 5.2%) and moving (7.1 ± 2.4%). These results can be used for the development of breeding programs using technologically advanced reproductive procedures in domestic and wild cats. https://doi.org/10.54680/fr24110110212.PMID:38538371 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: S Panyaboriban N Kupthammasan K Madsri N Mukem S Tarngwiriyakul P Khirilak P Panichayupakaranant M Wittayarat Source Type: research
Comparison of different freezing methods for micro-volume semen
CONCLUSION: For smaller (micro) volumes, the FLNV technique is better than the RFLN method. https://doi.org/10.54680/fr24110110412.PMID:38538372 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: J Liu Y H Li Y H Zhou X X Wang L X Tong H H Wang Source Type: research
Viscoelastic properties of decellularized and freeze-dried human dermis between i909c and 40cC
CONCLUSION: Freeze-dried ADM was protected in the glassy carbohydrate matrix. DMA observed two viscoelastic relaxations (i.e., alpha process at -20 degree C and beta process at -60 degree C). Overall changes in G' and G'' of freeze-dried ADM were relatively small within one order of magnitude between -90 degree C and 40 degree C. https://doi.org/10.54680/fr24110110612.PMID:38538373 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: Q P Jia S L Huang Y T Tang W Q Sun Source Type: research
The effect of different concentrations of laminarin on the quality of cryopreserved ram semen
CONCLUSION: The addition of 800 ug/mL laminarin to the freezing extender increases motility, viability, SOD activity, and plasma membrane integrity, while reducing abnormality and MDA production in freeze-thawed ram semen. https://doi.org/10.54680/fr24110110812.PMID:38538374 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: N Zangishhi H Hajarian H Karamishabankareh L Soltani Source Type: research
The comet assay as a method for assessing dna damage in cryopreserved samples
Cryo Letters. 2024 Jan-Feb;45(1):1-15.ABSTRACTThe preservation of the nuclear genome's integrity is paramount for the viability and overall health of cells, tissues, and organisms. DNA, being susceptible to damage under physiological conditions and vulnerable to both endogenous and environmental factors, faces constant threats. To assess DNA damage and repair within individual eukaryotic cells, the comet assay presents itself as a versatile, gel electrophoresis-based, relatively simple, and highly sensitive method. Originally designed to monitor DNA damage and repair within populations of mammalian cells, the comet assay h...
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: B P Plitta-Michalak A Ramos D Stepien M Trusiak M Michalak Source Type: research
Dog sperm cryopreservation using cryovials and different dilution steps
CONCLUSION: The sperm frozen using cryovials, one step dilution and the deep freezer (Group 2) proved to be a simple and suitable cryopreservation method for dog sperm. https://doi.org/10.54680/fr24110110312.PMID:38538368 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: S Ibrahim N A Hamad Talha J Cho Y Jeon I J Yu Source Type: research
Effects of stearic acid on the embryo cryopreservation in mouse
CONCLUSION: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.PMID:38538369 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: T N Igonina T A Rakhmanova A N Omelchenko K A Okotrub E Yu Brusentsev I N Rozhkova S Ya Amstislavsky Source Type: research
Reduced sperm number of murrah (Bubalus bubalis) bull semen in french mini-straw affects kinetic and functional competence after cryopreservation
CONCLUSION: The reduction of sperm concentrations to < 10 million/mL affects post-thaw Buffalo sperm kinetic and functional attributes.. https://doi.org/10.54680/fr24110110712.PMID:38538370 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: H P Yadav T K Mohanty R K Dewry S A Lone S Nath M Bhakat R K Baithalu S Tiwari D K Swain P Kumar A K Mohanty T K Datta Source Type: research
Supplementation of extender with chamuangone-enriched Garcinia cowa leaf extract improves quality parameters and longevity of cold-stored cat semen
CONCLUSION: 100 µg/mL chamuangone can improve sperm characteristics during 15 days of preservation at 4 degree C, keeping sperm alive (49.3 ± 5.2%) and moving (7.1 ± 2.4%). These results can be used for the development of breeding programs using technologically advanced reproductive procedures in domestic and wild cats. https://doi.org/10.54680/fr24110110212.PMID:38538371 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: S Panyaboriban N Kupthammasan K Madsri N Mukem S Tarngwiriyakul P Khirilak P Panichayupakaranant M Wittayarat Source Type: research
Comparison of different freezing methods for micro-volume semen
CONCLUSION: For smaller (micro) volumes, the FLNV technique is better than the RFLN method. https://doi.org/10.54680/fr24110110412.PMID:38538372 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: J Liu Y H Li Y H Zhou X X Wang L X Tong H H Wang Source Type: research
Viscoelastic properties of decellularized and freeze-dried human dermis between i909c and 40cC
CONCLUSION: Freeze-dried ADM was protected in the glassy carbohydrate matrix. DMA observed two viscoelastic relaxations (i.e., alpha process at -20 degree C and beta process at -60 degree C). Overall changes in G' and G'' of freeze-dried ADM were relatively small within one order of magnitude between -90 degree C and 40 degree C. https://doi.org/10.54680/fr24110110612.PMID:38538373 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: Q P Jia S L Huang Y T Tang W Q Sun Source Type: research
The effect of different concentrations of laminarin on the quality of cryopreserved ram semen
CONCLUSION: The addition of 800 ug/mL laminarin to the freezing extender increases motility, viability, SOD activity, and plasma membrane integrity, while reducing abnormality and MDA production in freeze-thawed ram semen. https://doi.org/10.54680/fr24110110812.PMID:38538374 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: N Zangishhi H Hajarian H Karamishabankareh L Soltani Source Type: research
The technology in cryotechnology
Cryo Letters. 2023 Nov-Dec;44(6):314-326.ABSTRACTThe process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or ...
Source: Cryo-Letters - February 5, 2024 Category: Biology Authors: J Hagedorn G Alkurdi S-A Barker R Brunotte T Deeb O Hubenia D Khayyat S Leal-Marin T Rittinghaus B Glasmacher Source Type: research
IGF-1 stabilizes goat sperm mitochondrial transmembrane potential and reduces dna fragmentation
CONCLUSION: IGF-1 may be used to improve post-thaw semen quality and fertility as measured by actual kidding rate. Doi.org/10.54680/fr23610110312.PMID:38311926 (Source: Cryo-Letters)
Source: Cryo-Letters - February 5, 2024 Category: Biology Authors: R Ranjan K Sharma M Kumar D K Swain S P Singh S D Kharche M K Singh M S Chauhan Source Type: research
Application of d cryo-plate technique for the cryopreservation of in vitro-grown shoot tips of Tarenaya spinosa (Cleomaceae)
CONCLUSION: The plants developed after cryopreservation maintained their in vitro multiplication capacity and demonstrated the efficiency of long-term conservation by D cryo-plate technique for T. spinosa. Doi.org/10.54680/fr23610110512.PMID:38311927 (Source: Cryo-Letters)
Source: Cryo-Letters - February 5, 2024 Category: Biology Authors: A F R M Vilardo G D Silva Lima L S Cordeiro N Albarello C Simoes-Gurgel Source Type: research
