A novel vitrified cryopreservation approach with stem cell-laden hydrogel microcapsules
CONCLUSION: Cell laden hydrogel microcapsules may have the potential to be the basis of a new strategy of cell cryopreservation and applications. https://doi.org/10.54680/fr24210110212.PMID:38557990 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: T Song B Liu Source Type: research
Cryopreservation and assessment of genetic fidelity Acorus calamus Linn., an endangered medicinal plant
CONCLUSION: Shoot tips of A. calamus that were cryopreserved had 88.3% regrowth using V cryo-plate technique and the regerants retained genetic fidelity. https://doi.org/10.54680/fr24210110412.PMID:38557991 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: K Parasher C Sharma S Sharma EmptyYN Y Shrishti P Mukherjee Source Type: research
Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research
CONCLUSION: Our findings suggest that a cryopreservation medium containing 0.3 M glucose can contribute to the safeguarding C. laucha rodent semen. https://doi.org/10.54680/fr24210110612.PMID:38557992 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: C D Corcini J Vilela N Gatti B Mion N Castro R T Franca A S Varela Junior Source Type: research
Targeted development and optimization of small-molecule ice recrystallization inhibitors (IRIs) for the cryopreservation of biological systems
This article provides a detailed overview of the development of small-molecule carbohydrate-based IRIs and highlights the crucial cell-specific biological considerations that must be taken into account when assessing cryopreservation outcomes. https://doi.org/10.54680/fr24210110112.PMID:38557986 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: L E McMunn E M Walsh R N Ben Source Type: research
RNA-Seq analysis reveals the mechanism in response to cold stress of peach cv 'Dingjiaba'
CONCLUSIONS: These results provide novel insights into a series of molecular mechanisms underlying physiological metabolism and defense. https://doi.org/10.54680/fr24210110312.PMID:38557987 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: X Li H Wang X Wu Source Type: research
The kinetics of goat sperm is negatively affected after freezing in an extender including zinc oxide nanoparticles
CONCLUSION: The addition of Np-ZnO (200 ug/mL) to the goat semen freezing extender improved the total motility of cells, whilst negatively affecting sperm kinetics. https://doi.org/10.54680/fr24210110512.PMID:38557988 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: L C Pereira Arruda G de Oliveira Alves Pinto G F Carneiro M M Pessoa Guerra Source Type: research
Testing of arctic insect hemolymph as a secondary agent in applied cryopreservation
CONCLUSION: Although the use of insect hemolymph as a complementary agent in applied cryopreservation is a problem in terms of practical application, such studies could initiate new trends in the search for the most successful hemolymph-like cryoprotectant systems. https://doi.org/10.54680/fr24210110712.PMID:38557989 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: N G Li Source Type: research
A novel vitrified cryopreservation approach with stem cell-laden hydrogel microcapsules
CONCLUSION: Cell laden hydrogel microcapsules may have the potential to be the basis of a new strategy of cell cryopreservation and applications. https://doi.org/10.54680/fr24210110212.PMID:38557990 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: T Song B Liu Source Type: research
Cryopreservation and assessment of genetic fidelity Acorus calamus Linn., an endangered medicinal plant
CONCLUSION: Shoot tips of A. calamus that were cryopreserved had 88.3% regrowth using V cryo-plate technique and the regerants retained genetic fidelity. https://doi.org/10.54680/fr24210110412.PMID:38557991 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: K Parasher C Sharma S Sharma EmptyYN Y Shrishti P Mukherjee Source Type: research
Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research
CONCLUSION: Our findings suggest that a cryopreservation medium containing 0.3 M glucose can contribute to the safeguarding C. laucha rodent semen. https://doi.org/10.54680/fr24210110612.PMID:38557992 (Source: Cryo-Letters)
Source: Cryo-Letters - April 1, 2024 Category: Biology Authors: C D Corcini J Vilela N Gatti B Mion N Castro R T Franca A S Varela Junior Source Type: research
The comet assay as a method for assessing dna damage in cryopreserved samples
Cryo Letters. 2024 Jan-Feb;45(1):1-15.ABSTRACTThe preservation of the nuclear genome's integrity is paramount for the viability and overall health of cells, tissues, and organisms. DNA, being susceptible to damage under physiological conditions and vulnerable to both endogenous and environmental factors, faces constant threats. To assess DNA damage and repair within individual eukaryotic cells, the comet assay presents itself as a versatile, gel electrophoresis-based, relatively simple, and highly sensitive method. Originally designed to monitor DNA damage and repair within populations of mammalian cells, the comet assay h...
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: B P Plitta-Michalak A Ramos D Stepien M Trusiak M Michalak Source Type: research
Dog sperm cryopreservation using cryovials and different dilution steps
CONCLUSION: The sperm frozen using cryovials, one step dilution and the deep freezer (Group 2) proved to be a simple and suitable cryopreservation method for dog sperm. https://doi.org/10.54680/fr24110110312.PMID:38538368 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: S Ibrahim N A Hamad Talha J Cho Y Jeon I J Yu Source Type: research
Effects of stearic acid on the embryo cryopreservation in mouse
CONCLUSION: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.PMID:38538369 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: T N Igonina T A Rakhmanova A N Omelchenko K A Okotrub E Yu Brusentsev I N Rozhkova S Ya Amstislavsky Source Type: research
Reduced sperm number of murrah (Bubalus bubalis) bull semen in french mini-straw affects kinetic and functional competence after cryopreservation
CONCLUSION: The reduction of sperm concentrations to < 10 million/mL affects post-thaw Buffalo sperm kinetic and functional attributes.. https://doi.org/10.54680/fr24110110712.PMID:38538370 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: H P Yadav T K Mohanty R K Dewry S A Lone S Nath M Bhakat R K Baithalu S Tiwari D K Swain P Kumar A K Mohanty T K Datta Source Type: research
Supplementation of extender with chamuangone-enriched Garcinia cowa leaf extract improves quality parameters and longevity of cold-stored cat semen
CONCLUSION: 100 µg/mL chamuangone can improve sperm characteristics during 15 days of preservation at 4 degree C, keeping sperm alive (49.3 ± 5.2%) and moving (7.1 ± 2.4%). These results can be used for the development of breeding programs using technologically advanced reproductive procedures in domestic and wild cats. https://doi.org/10.54680/fr24110110212.PMID:38538371 (Source: Cryo-Letters)
Source: Cryo-Letters - March 27, 2024 Category: Biology Authors: S Panyaboriban N Kupthammasan K Madsri N Mukem S Tarngwiriyakul P Khirilak P Panichayupakaranant M Wittayarat Source Type: research
