N-Glycosylation-dependent regulation of immune-specific tetraspanins CD37 and CD53
In this study we investigated the effects of glycosylation of CD37 and CD53, two tetraspanins important for cellular and humoral immunity. Broad and cell-specific repertoires of N-glycosylated CD37 and CD53 were observed in human B cells. We generated different glycosylation mutants of CD37 and CD53 and analyzed their localization, nanoscale plasma membrane organization and partner protein interaction capacity. Abrogation of glycosylation in CD37 revealed the importance of this modification for CD37 surface expression, whereas surface expression of CD53 was unaffected by its glycosylation. Single-molecule dSTORM microscopy...
Source: Biophysical Journal - November 30, 2023 Category: Physics Authors: Sjoerd van Deventer Ilse A Hoogvliet Merel van de Voort Frank Arnold Martin Ter Beest Annemiek B van Spriel Source Type: research
N-Glycosylation-dependent regulation of immune-specific tetraspanins CD37 and CD53
In this study we investigated the effects of glycosylation of CD37 and CD53, two tetraspanins important for cellular and humoral immunity. Broad and cell-specific repertoires of N-glycosylated CD37 and CD53 were observed in human B cells. We generated different glycosylation mutants of CD37 and CD53 and analyzed their localization, nanoscale plasma membrane organization and partner protein interaction capacity. Abrogation of glycosylation in CD37 revealed the importance of this modification for CD37 surface expression, whereas surface expression of CD53 was unaffected by its glycosylation. Single-molecule dSTORM microscopy...
Source: Biophysical Journal - November 30, 2023 Category: Physics Authors: Sjoerd van Deventer Ilse A Hoogvliet Merel van de Voort Frank Arnold Martin Ter Beest Annemiek B van Spriel Source Type: research
Membrane extraction in native lipid nanodiscs reveals dynamic regulation of Cdc42 complexes during cell polarization
Biophys J. 2023 Nov 23:S0006-3495(23)00721-X. doi: 10.1016/j.bpj.2023.11.021. Online ahead of print.ABSTRACTEmbryonic development requires the establishment of cell polarity to enable cell fate segregation and tissue morphogenesis. This process is regulated by Par complex proteins, which partition into polarized membrane domains and direct downstream polarized cell behaviors. The kinase aPKC (along with its cofactor Par6) is a key member of this network and can be recruited to the plasma membrane by either the small GTPase Cdc42 or the scaffolding protein Par3. Although in vitro interactions among these proteins are well e...
Source: Biophysical Journal - November 25, 2023 Category: Physics Authors: Lars N Deutz Sena Sar ıkaya Daniel J Dickinson Source Type: research
Molecular insight into how the position of an abasic site modifies DNA duplex stability and dynamics
Biophys J. 2023 Nov 23:S0006-3495(23)00722-1. doi: 10.1016/j.bpj.2023.11.022. Online ahead of print.ABSTRACTLocal perturbations to DNA base-pairing stability from lesions and chemical modifications can alter the stability and dynamics of an entire oligonucleotide. End effects may cause the position of a disruption within a short duplex to influence duplex stability and structural dynamics, yet this aspect of nucleic acid modifications is often overlooked. We investigate how the position of an abasic site (AP site) impacts the stability and dynamics of short DNA duplexes. Using a combination of steady-state and time-resolve...
Source: Biophysical Journal - November 25, 2023 Category: Physics Authors: Brennan Ashwood Michael S Jones Yumin Lee Joseph R Sachleben Andrew L Ferguson Andrei Tokmakoff Source Type: research
Multiscale biophysical models of cardiomyopathies reveal complexities challenging existing dogmas
Biophys J. 2023 Nov 24:S0006-3495(23)00714-2. doi: 10.1016/j.bpj.2023.11.014. Online ahead of print.ABSTRACTMutations in sarcomeric proteins, including myosin, cause a variety of cardiomyopathies. A prominent hypothesis has been that myosin mutations causing hypercontractility of the motor lead to hypertrophic cardiomyopathy, while those causing hypocontractility lead to dilated cardiomyopathy; however, recent biophysical studies using multiscale computational and experimental models have revealed complexities not captured by this hypothesis. We summarize recent publications in Biophysical Journal challenging this dogma an...
Source: Biophysical Journal - November 25, 2023 Category: Physics Authors: Brent Scott Michael J Greenberg Source Type: research
