American Journal of Physiology. Cell Physiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

The myokine CCL5 recruits subcutaneous preadipocytes and promotes intramuscular fat deposition in obese mice
In this study, it was discovered that the regulation of IMF levels is achieved through the chemokine (C-C motif) ligand 5 (CCL5)/chemokine (C-C motif) receptor 5 (CCR5) pathway by modulating adipocyte migration. In coculture experiments, C2C12 myotubes were more effective in promoting the migration of 3T3-L1 preadipocytes than C2C12 myoblasts, along with increasing CCL5. Correspondingly, overexpressing the CCR5, one of the receptors of CCL5, in 3T3-L1 preadipocytes facilitated their migration. Conversely, the application of the CCL5/CCR5 inhibitor, MARAVIROC (MVC), reduced this migration. In vivo, transplanted experiments ...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Wensai Yu Yao Yao Nanwei Ye Yuelei Zhao Zijian Ye Wei Wei Lifan Zhang Jie Chen Source Type: research

TIMP2 protects against sepsis-associated acute kidney injury by cAMP/NLRP3 axis-mediated pyroptosis
In this study, we investigated the impact of kidney tubule-specific Timp2 knockout mice on kidney injury and inflammation. Our findings demonstrated that Timp2-knockout mice exhibited more severe kidney injury than wild-type mice, along with elevated levels of pyroptosis markers NLRP3, Caspase1, and GSDMD in the early stage of SA-AKI. Conversely, the expression of exogenous TIMP2 in TIMP2-knockout mice still protected against kidney damage and inflammation. In vitro experiments, using recombinant TIMP2 protein, TIMP2 knockdown demonstrated that exogenous TIMP2 inhibited pyroptosis of renal tubular cells stimulated by LPS. ...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Dongxue Xu Jun Jiang Ye Liu Jingjing Pang Jinmeng Suo Yiming Li Zhiyong Peng Source Type: research

A novel approach to determine the critical survival threshold of cellular oxygen within spheroids via integrating live/dead cell imaging with oxygen modeling
In this study, we introduce a non-invasive, high-throughput methodology to quantify critical survival partial oxygen pressure (pO₂) with high spatial resolution within spheroids by employing a combination of controlled hypoxic conditions, semi-automated live/dead cell imaging, and computational oxygen modeling. The oxygen-permeable, micro-pyramid patterned culture plates created a precisely controlled oxygen condition around the individual spheroid. Live/dead cell imaging provided the geometric information of the live/dead boundary within spheroids. Finally, computational oxygen modeling calculated the pO₂ at the live/...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Kuang-Ming Shang Hiroyuki Kato Nelson Gonzalez Fouad Kandeel Yu-Chong Tai Hirotake Komatsu Source Type: research

Roles for integrin α3β1 in regulating the ability of epithelial cells to modulate the microenvironment during normal and pathological tissue remodeling
Am J Physiol Cell Physiol. 2024 Mar 18. doi: 10.1152/ajpcell.00128.2024. Online ahead of print.ABSTRACTIntegrin receptors for the extracellular matrix activate intracellular signaling pathways that are critical for tissue development, homeostasis, and regeneration/repair, and their loss or dysregulation contributes to many developmental defects and tissue pathologies. This review will focus on tissue remodeling roles for integrin α3β1, a receptor for laminins found in the basement membranes that underlie epithelial cell layers. As a paradigm, we will discuss literature that supports a role for α3β1 in promoting ability...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Rakshitha Pandulal Miskin C Michael DiPersio Source Type: research

Kynurenine 3-monooxygenase (KMO) limits < em > de novo < /em > NAD < sup > + < /sup > synthesis through dietary tryptophan in renal proximal tubule epithelial cell models
Am J Physiol Cell Physiol. 2024 Mar 18. doi: 10.1152/ajpcell.00445.2023. Online ahead of print.ABSTRACTNicotinamide adenine dinucleotide (NAD+) is a pivotal coenzyme, essential for cellular reactions, metabolism, and mitochondrial function. Depletion of kidney NAD+ levels and reduced de novo NAD+ synthesis through the tryptophan-kynurenine pathway are linked to acute kidney injury (AKI), while augmenting NAD+ shows promise in reducing AKI. We investigated de novo NAD+ biosynthesis using in vitro, ex vivo and in vivo models to understand its role in AKI. 2D cultures of human primary renal proximal tubule epithelial cells (R...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Yougang Zhai Jose A Chavez Katharine E D'Aquino Rong Meng Andrea R Nawrocki Alessandro Pocai Lifeng Wang Li-Jun Ma Source Type: research

The myokine CCL5 recruits subcutaneous preadipocytes and promotes intramuscular fat deposition in obese mice
In this study, it was discovered that the regulation of IMF levels is achieved through the chemokine (C-C motif) ligand 5 (CCL5)/chemokine (C-C motif) receptor 5 (CCR5) pathway by modulating adipocyte migration. In coculture experiments, C2C12 myotubes were more effective in promoting the migration of 3T3-L1 preadipocytes than C2C12 myoblasts, along with increasing CCL5. Correspondingly, overexpressing the CCR5, one of the receptors of CCL5, in 3T3-L1 preadipocytes facilitated their migration. Conversely, the application of the CCL5/CCR5 inhibitor, MARAVIROC (MVC), reduced this migration. In vivo, transplanted experiments ...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Wensai Yu Yao Yao Nanwei Ye Yuelei Zhao Zijian Ye Wei Wei Lifan Zhang Jie Chen Source Type: research

TIMP2 protects against sepsis-associated acute kidney injury by cAMP/NLRP3 axis-mediated pyroptosis
In this study, we investigated the impact of kidney tubule-specific Timp2 knockout mice on kidney injury and inflammation. Our findings demonstrated that Timp2-knockout mice exhibited more severe kidney injury than wild-type mice, along with elevated levels of pyroptosis markers NLRP3, Caspase1, and GSDMD in the early stage of SA-AKI. Conversely, the expression of exogenous TIMP2 in TIMP2-knockout mice still protected against kidney damage and inflammation. In vitro experiments, using recombinant TIMP2 protein, TIMP2 knockdown demonstrated that exogenous TIMP2 inhibited pyroptosis of renal tubular cells stimulated by LPS. ...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Dongxue Xu Jun Jiang Ye Liu Jingjing Pang Jinmeng Suo Yiming Li Zhiyong Peng Source Type: research

A novel approach to determine the critical survival threshold of cellular oxygen within spheroids via integrating live/dead cell imaging with oxygen modeling
In this study, we introduce a non-invasive, high-throughput methodology to quantify critical survival partial oxygen pressure (pO₂) with high spatial resolution within spheroids by employing a combination of controlled hypoxic conditions, semi-automated live/dead cell imaging, and computational oxygen modeling. The oxygen-permeable, micro-pyramid patterned culture plates created a precisely controlled oxygen condition around the individual spheroid. Live/dead cell imaging provided the geometric information of the live/dead boundary within spheroids. Finally, computational oxygen modeling calculated the pO₂ at the live/...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Kuang-Ming Shang Hiroyuki Kato Nelson Gonzalez Fouad Kandeel Yu-Chong Tai Hirotake Komatsu Source Type: research

Roles for integrin α3β1 in regulating the ability of epithelial cells to modulate the microenvironment during normal and pathological tissue remodeling
Am J Physiol Cell Physiol. 2024 Mar 18. doi: 10.1152/ajpcell.00128.2024. Online ahead of print.ABSTRACTIntegrin receptors for the extracellular matrix activate intracellular signaling pathways that are critical for tissue development, homeostasis, and regeneration/repair, and their loss or dysregulation contributes to many developmental defects and tissue pathologies. This review will focus on tissue remodeling roles for integrin α3β1, a receptor for laminins found in the basement membranes that underlie epithelial cell layers. As a paradigm, we will discuss literature that supports a role for α3β1 in promoting ability...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Rakshitha Pandulal Miskin C Michael DiPersio Source Type: research

Kynurenine 3-monooxygenase (KMO) limits < em > de novo < /em > NAD < sup > + < /sup > synthesis through dietary tryptophan in renal proximal tubule epithelial cell models
Am J Physiol Cell Physiol. 2024 Mar 18. doi: 10.1152/ajpcell.00445.2023. Online ahead of print.ABSTRACTNicotinamide adenine dinucleotide (NAD+) is a pivotal coenzyme, essential for cellular reactions, metabolism, and mitochondrial function. Depletion of kidney NAD+ levels and reduced de novo NAD+ synthesis through the tryptophan-kynurenine pathway are linked to acute kidney injury (AKI), while augmenting NAD+ shows promise in reducing AKI. We investigated de novo NAD+ biosynthesis using in vitro, ex vivo and in vivo models to understand its role in AKI. 2D cultures of human primary renal proximal tubule epithelial cells (R...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Yougang Zhai Jose A Chavez Katharine E D'Aquino Rong Meng Andrea R Nawrocki Alessandro Pocai Lifeng Wang Li-Jun Ma Source Type: research

The myokine CCL5 recruits subcutaneous preadipocytes and promotes intramuscular fat deposition in obese mice
In this study, it was discovered that the regulation of IMF levels is achieved through the chemokine (C-C motif) ligand 5 (CCL5)/chemokine (C-C motif) receptor 5 (CCR5) pathway by modulating adipocyte migration. In coculture experiments, C2C12 myotubes were more effective in promoting the migration of 3T3-L1 preadipocytes than C2C12 myoblasts, along with increasing CCL5. Correspondingly, overexpressing the CCR5, one of the receptors of CCL5, in 3T3-L1 preadipocytes facilitated their migration. Conversely, the application of the CCL5/CCR5 inhibitor, MARAVIROC (MVC), reduced this migration. In vivo, transplanted experiments ...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Wensai Yu Yao Yao Nanwei Ye Yuelei Zhao Zijian Ye Wei Wei Lifan Zhang Jie Chen Source Type: research

TIMP2 protects against sepsis-associated acute kidney injury by cAMP/NLRP3 axis-mediated pyroptosis
In this study, we investigated the impact of kidney tubule-specific Timp2 knockout mice on kidney injury and inflammation. Our findings demonstrated that Timp2-knockout mice exhibited more severe kidney injury than wild-type mice, along with elevated levels of pyroptosis markers NLRP3, Caspase1, and GSDMD in the early stage of SA-AKI. Conversely, the expression of exogenous TIMP2 in TIMP2-knockout mice still protected against kidney damage and inflammation. In vitro experiments, using recombinant TIMP2 protein, TIMP2 knockdown demonstrated that exogenous TIMP2 inhibited pyroptosis of renal tubular cells stimulated by LPS. ...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Dongxue Xu Jun Jiang Ye Liu Jingjing Pang Jinmeng Suo Yiming Li Zhiyong Peng Source Type: research

A novel approach to determine the critical survival threshold of cellular oxygen within spheroids via integrating live/dead cell imaging with oxygen modeling
In this study, we introduce a non-invasive, high-throughput methodology to quantify critical survival partial oxygen pressure (pO₂) with high spatial resolution within spheroids by employing a combination of controlled hypoxic conditions, semi-automated live/dead cell imaging, and computational oxygen modeling. The oxygen-permeable, micro-pyramid patterned culture plates created a precisely controlled oxygen condition around the individual spheroid. Live/dead cell imaging provided the geometric information of the live/dead boundary within spheroids. Finally, computational oxygen modeling calculated the pO₂ at the live/...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Kuang-Ming Shang Hiroyuki Kato Nelson Gonzalez Fouad Kandeel Yu-Chong Tai Hirotake Komatsu Source Type: research

Roles for integrin α3β1 in regulating the ability of epithelial cells to modulate the microenvironment during normal and pathological tissue remodeling
Am J Physiol Cell Physiol. 2024 Mar 18. doi: 10.1152/ajpcell.00128.2024. Online ahead of print.ABSTRACTIntegrin receptors for the extracellular matrix activate intracellular signaling pathways that are critical for tissue development, homeostasis, and regeneration/repair, and their loss or dysregulation contributes to many developmental defects and tissue pathologies. This review will focus on tissue remodeling roles for integrin α3β1, a receptor for laminins found in the basement membranes that underlie epithelial cell layers. As a paradigm, we will discuss literature that supports a role for α3β1 in promoting ability...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Rakshitha Pandulal Miskin C Michael DiPersio Source Type: research

Kynurenine 3-monooxygenase (KMO) limits < em > de novo < /em > NAD < sup > + < /sup > synthesis through dietary tryptophan in renal proximal tubule epithelial cell models
Am J Physiol Cell Physiol. 2024 Mar 18. doi: 10.1152/ajpcell.00445.2023. Online ahead of print.ABSTRACTNicotinamide adenine dinucleotide (NAD+) is a pivotal coenzyme, essential for cellular reactions, metabolism, and mitochondrial function. Depletion of kidney NAD+ levels and reduced de novo NAD+ synthesis through the tryptophan-kynurenine pathway are linked to acute kidney injury (AKI), while augmenting NAD+ shows promise in reducing AKI. We investigated de novo NAD+ biosynthesis using in vitro, ex vivo and in vivo models to understand its role in AKI. 2D cultures of human primary renal proximal tubule epithelial cells (R...
Source: American Journal of Physiology. Cell Physiology - March 18, 2024 Category: Cytology Authors: Yougang Zhai Jose A Chavez Katharine E D'Aquino Rong Meng Andrea R Nawrocki Alessandro Pocai Lifeng Wang Li-Jun Ma Source Type: research