Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00903. Online ahead of print.ABSTRACTPituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos w...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: D óra Török Bence Somosk ői Lilla Bord ás D óra Reglődi S ándor Cseh Source Type: research
Multilocus sequence analysis (MLSA) of a Nocardia cyriacigeorgica strain causing severe bovine mastitis in Bosnia and Herzegovina
We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the s...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Oliver Stevanovi ć Dubravka Milanov Isidora Pro šić Vladimir Gajdov Drago Nedi ć Željko Sladojević Andrea Radalj Source Type: research
Plasma melatonin concentration during the early post-partum period in Thoroughbred mares and their foals
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00883. Online ahead of print.ABSTRACTThe authors aimed to determine the plasma melatonin concentration in mares and their new-born foals in the early post-partum period. Blood samples were collected from the jugular vein of 53 mare-foal pairs within twelve hours after parturition. Plasma melatonin levels were measured by ELISA. The melatonin concentration, adjusted for the moment of parturition using a generalised linear model, was 34.58 pg mL-1 in mares. It was significantly lower (27.63 pg mL-1) in the new-born foals. However, the melatonin concentration declined different...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Andr ás Gáspárdy Gemma Gallagher Bor óka Bartha S ándor Cseh S ándor György Fekete Bence Somosk ői Source Type: research
Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00903. Online ahead of print.ABSTRACTPituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos w...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: D óra Török Bence Somosk ői Lilla Bord ás D óra Reglődi S ándor Cseh Source Type: research
Multilocus sequence analysis (MLSA) of a Nocardia cyriacigeorgica strain causing severe bovine mastitis in Bosnia and Herzegovina
We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the s...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Oliver Stevanovi ć Dubravka Milanov Isidora Pro šić Vladimir Gajdov Drago Nedi ć Željko Sladojević Andrea Radalj Source Type: research
Plasma melatonin concentration during the early post-partum period in Thoroughbred mares and their foals
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00883. Online ahead of print.ABSTRACTThe authors aimed to determine the plasma melatonin concentration in mares and their new-born foals in the early post-partum period. Blood samples were collected from the jugular vein of 53 mare-foal pairs within twelve hours after parturition. Plasma melatonin levels were measured by ELISA. The melatonin concentration, adjusted for the moment of parturition using a generalised linear model, was 34.58 pg mL-1 in mares. It was significantly lower (27.63 pg mL-1) in the new-born foals. However, the melatonin concentration declined different...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Andr ás Gáspárdy Gemma Gallagher Bor óka Bartha S ándor Cseh S ándor György Fekete Bence Somosk ői Source Type: research
Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00903. Online ahead of print.ABSTRACTPituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos w...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: D óra Török Bence Somosk ői Lilla Bord ás D óra Reglődi S ándor Cseh Source Type: research
Multilocus sequence analysis (MLSA) of a Nocardia cyriacigeorgica strain causing severe bovine mastitis in Bosnia and Herzegovina
We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the s...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Oliver Stevanovi ć Dubravka Milanov Isidora Pro šić Vladimir Gajdov Drago Nedi ć Željko Sladojević Andrea Radalj Source Type: research
Plasma melatonin concentration during the early post-partum period in Thoroughbred mares and their foals
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00883. Online ahead of print.ABSTRACTThe authors aimed to determine the plasma melatonin concentration in mares and their new-born foals in the early post-partum period. Blood samples were collected from the jugular vein of 53 mare-foal pairs within twelve hours after parturition. Plasma melatonin levels were measured by ELISA. The melatonin concentration, adjusted for the moment of parturition using a generalised linear model, was 34.58 pg mL-1 in mares. It was significantly lower (27.63 pg mL-1) in the new-born foals. However, the melatonin concentration declined different...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Andr ás Gáspárdy Gemma Gallagher Bor óka Bartha S ándor Cseh S ándor György Fekete Bence Somosk ői Source Type: research
Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00903. Online ahead of print.ABSTRACTPituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos w...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: D óra Török Bence Somosk ői Lilla Bord ás D óra Reglődi S ándor Cseh Source Type: research
Multilocus sequence analysis (MLSA) of a Nocardia cyriacigeorgica strain causing severe bovine mastitis in Bosnia and Herzegovina
We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the s...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Oliver Stevanovi ć Dubravka Milanov Isidora Pro šić Vladimir Gajdov Drago Nedi ć Željko Sladojević Andrea Radalj Source Type: research
Plasma melatonin concentration during the early post-partum period in Thoroughbred mares and their foals
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00883. Online ahead of print.ABSTRACTThe authors aimed to determine the plasma melatonin concentration in mares and their new-born foals in the early post-partum period. Blood samples were collected from the jugular vein of 53 mare-foal pairs within twelve hours after parturition. Plasma melatonin levels were measured by ELISA. The melatonin concentration, adjusted for the moment of parturition using a generalised linear model, was 34.58 pg mL-1 in mares. It was significantly lower (27.63 pg mL-1) in the new-born foals. However, the melatonin concentration declined different...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Andr ás Gáspárdy Gemma Gallagher Bor óka Bartha S ándor Cseh S ándor György Fekete Bence Somosk ői Source Type: research
Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00903. Online ahead of print.ABSTRACTPituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos w...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: D óra Török Bence Somosk ői Lilla Bord ás D óra Reglődi S ándor Cseh Source Type: research
Multilocus sequence analysis (MLSA) of a Nocardia cyriacigeorgica strain causing severe bovine mastitis in Bosnia and Herzegovina
We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the s...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Oliver Stevanovi ć Dubravka Milanov Isidora Pro šić Vladimir Gajdov Drago Nedi ć Željko Sladojević Andrea Radalj Source Type: research
Plasma melatonin concentration during the early post-partum period in Thoroughbred mares and their foals
Acta Vet Hung. 2023 Sep 6. doi: 10.1556/004.2023.00883. Online ahead of print.ABSTRACTThe authors aimed to determine the plasma melatonin concentration in mares and their new-born foals in the early post-partum period. Blood samples were collected from the jugular vein of 53 mare-foal pairs within twelve hours after parturition. Plasma melatonin levels were measured by ELISA. The melatonin concentration, adjusted for the moment of parturition using a generalised linear model, was 34.58 pg mL-1 in mares. It was significantly lower (27.63 pg mL-1) in the new-born foals. However, the melatonin concentration declined different...
Source: Acta Veterinaria Hungarica - September 7, 2023 Category: Veterinary Research Authors: Andr ás Gáspárdy Gemma Gallagher Bor óka Bartha S ándor Cseh S ándor György Fekete Bence Somosk ői Source Type: research
