Molecular characterisation of Mycoplasma gallisepticum isolates from Iran in the period 2012-2017.
Abstract Mycoplasma gallisepticum (MG) causes chronic non-fatal diseases in the poultry industry with a remarkable decrease in feed consumption, egg production and other production indices. To study the genetic characteristics of MG isolates in commercial and backyard poultry flocks, 21 positive samples from different regions of Iran were analysed in the period 2012-2017. Typical macroscopic and histopathological lesions of the upper respiratory tract and lungs were found, similar to those reported by other researchers. A 298-361 bp part of the mgc2 gene was sequenced and analysed. Based on the phylogenetic analys...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Norouzian H, Farjanikish G, Hosseini H Tags: Acta Vet Hung Source Type: research

The use of inflammatory markers as a diagnostic and prognostic approach in neonatal calves with septicaemia.
The objective of this study was to evaluate the usefulness of inflammatory markers as a diagnostic and prognostic approach in neonatal calves with septicaemia. The study material consisted of 13 neonatal calves with septicaemia (septicaemic calves, SC) and ten healthy neonatal calves (control calves, CC). Blood samples were collected for biochemical, haematological and microbiological analyses. In addition, faecal samples were collected for microbiological and virological analyses. Three of neonatal calves with septicaemia were positive for E. coli (E. coli O157 serotype) by microbiological examination, but all neonatal ca...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Kirbas A, Kandemir FM, Celebi D, Hanedan B, Timurkan MO Tags: Acta Vet Hung Source Type: research

Haematological and biochemical profiles during the puerperium in dairy cows - Short communication.
Abstract The main aim of the current study was to assess the prevalence of anaemia in Holstein dairy cows during the puerperium, and the haematological and biochemical profile of dairy cows with and without anaemia. The study was conducted in seven dairy herds in São Paulo State, Brazil. The evaluated sample comprised a total of 336 Holstein cows. Blood samples were collected at postpartum day 25 ± 3. Haematological analysis included white blood cell, red blood cell and platelet count, haematocrit value, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin and mean corpusc...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Paiano RB, Lahr FC, Silva LSB, Marques DS, Ferreira CA, Birgel DB, Bisinotto RS, Birgel Junior EH Tags: Acta Vet Hung Source Type: research

Relationship between RNASE1, ANG and RNASE6 gene polymorphism and the values of blood indices in suckling piglets.
Abstract The relationship between PcR-restriction fragment length polymorphism in RNASE1 (296 A/G), ANG (149 G/T) and RNASE6 (389 C/T) genes and the values of haematological and biochemical blood indices was analysed in crossbred suckling piglets (n = 473), aged 21 ± 3 days (younger, n = 274) and 35 ± 3 days (older, n = 199), descending from Polish Large White × Polish Landrace sows and Duroc × Pietrain boars. The observed distribution of all genotypes was consistent with the Hardy-Weinberg equilibrium. Anaemia was more common in younger piglets with RNASE1 GA genotype but in the blood of...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Szymańska H, Życzko K, Zabolewicz T Tags: Acta Vet Hung Source Type: research

Sarcocystis rileyi emerging in Hungary: is rice breast disease underreported in the region?
ute;r L, Hornok S Abstract Reports of Sarcocystis rileyi-like protozoa ('rice breast disease') from anseriform birds had been rare in Europe until the last two decades, when S. rileyi was identified in northern Europe and the UK. However, despite the economic losses resulting from S. rileyi infection, no recent accounts are available on its presence (which can be suspected) in most parts of central, western, southern and eastern Europe. Between 2014 and 2019, twelve mallards (Anas platyrhynchos) were observed to have rice breast disease in Hungary, and the last one of these 12 cases allowed molecular identificatio...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Szekeres S, Juhász A, Kondor M, Takács N, Sugár L, Hornok S Tags: Acta Vet Hung Source Type: research

Survey of lungworm infection of domestic cats in Hungary.
Abstract From 61 settlements of 12 Hungarian counties, 303 domestic cats were included in this survey. Between autumn 2016 and spring 2018, fresh faecal samples were randomly collected and examined by flotation and by the Baermann-Wetzel method for the presence of lungworm infection. No eggs of Eucoleus aerophilus were detected. Morphological identification of first instar larvae (L1) was also carried out. In the faeces of 60 cats (19.8%) from 17 settlements and Budapest, L1 of Aelurostrongylus abstrusus were found. More than half of the cats were from the western part of the country. The average number of larvae ...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Kiszely S, Gyurkovszky M, Solymosi N, Farkas R Tags: Acta Vet Hung Source Type: research

Alteration of avian hepatic cytochrome P450 gene expression and activity by certain feed additives.
kó G Abstract We investigated the effect of four feed additives, namely β-glucan, a drinking water acidifier (DWA), a sanguinarine-containing product (SN) and fulvic acid, on hepatic cytochrome P450 (CYP) mRNA expression and CYP enzyme activity in chickens. The test substances were given to the chickens in the recommended dose or in tenfold dose. The administration of 5 mg/kg body weight (bw) β-glucan and 0.1 ml/kg bw DWA for five days decreased the relative gene expression of CYP1A4 and CYP2C23a. The dosing of 50 mg/kg bw β-glucan, 5 and 50 mg/kg bw SN, 1 ml/kg bw DWA and 250 mg/kg bw fulvic...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Palócz O, Szita G, Csikó G Tags: Acta Vet Hung Source Type: research

Effect of three commercial extenders on sperm motility and fertility in liquid ram semen stored at 15 °C or 5 °C.
In conclusion, liquid storage at 5 °C with OVIX extender is an interesting option since non-animal components are used, and this extender offers similar in vitro and in vivo efficacy as other extenders containing animal components. PMID: 31549544 [PubMed - in process] (Source: Acta Veterinaria Hungarica)
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Arando A, Delgado JV, León JM, Nogales S, Navas-González FJ, Pizarro MG, Pérez-Marín CC Tags: Acta Vet Hung Source Type: research

First detection of heat shock protein 60 and 70 in the serum of early pregnant bitches.
n S Abstract Heat shock proteins (HSPs) belong to a group of cellular stress proteins. Heat shock protein 10 immunoregulates and promotes growth during early gestation in humans, while HSP70 is considered to regulate autophagy and apoptosis during pregnancy and parturition. Both HSPs are detectable in the serum and placentas of early pregnant women and considered to contribute to the establishment of pregnancy. Within this pilot study we aimed (1) to assess whether HSPs 10, 60 and 70 are measurable in the serum of healthy early pregnant and non-pregnant bitches, and (2) to explore whether measurable differences be...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Schäfer-Somi S, Aksoy OA, Ergene O, Darbaz I, Herkner KR, Aslan S Tags: Acta Vet Hung Source Type: research

Prevalence and characteristics of methicillin-resistant staphylococci in goats on the island of Tenerife, Spain.
uirre-Jaime A, Arias Á Abstract The aim of this study was to determine the prevalence of methicillin-resistant Staphylococcus (MRS) in healthy goats on the Island of Tenerife, Spain, as well as to identify the phenotypic and genotypic characteristics of the strains found. A cross-sectional prevalence study was conducted. A total of 158 goats from 15 different farms were sampled between September 2017 and January 2018. The percentage of positive samples of methicillin-resistant Staphylococcus aureus (MRSA) was 15.8% (25/158) and that of methicillin-resistant coagulase-negative staphylococci (MRCoNS) was 6.9%...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Abreu R, Rodríguez-Álvarez C, Lecuona M, Castro-Hernández B, González JC, Aguirre-Jaime A, Arias Á Tags: Acta Vet Hung Source Type: research

Changes in the values of two ultrasound-examined teat parameters during the dry period in dairy cows.
zsér J, Póti P Abstract The length of the streak canal and the area of the teat end were studied by ultrasound during the dry period in 40 Holstein-Friesian cows. In the first week, the values of these teat parameters decreased significantly (P
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Tóth T, Abonyi-Tóth Z, Pajor F, Kocsis R, Juhász A, Tőzsér J, Póti P Tags: Acta Vet Hung Source Type: research

Carbon source utilisation and evaluation of the Biolog system in the identification of Actinobacillus pleuropneumoniae.
, Fodor L Abstract Sixty-eight Actinobacillus pleuropneumoniae strains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014. A total of 41 isolates were identified as A. pleuropneumoniae bio-type I and 27 strains as biotype II based on cultural, morphological and biochemical characteristics. The aim of this study was to evaluate metabolic fingerprinting in the species-level identification of A. pleuropneumoniae isolates. Utilisation of carbon sources by these field isolates and six reference strains was characterised by the Biolog system (GN2 Microplate, Micr...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Makrai L, Sárközi R, Fodor L Tags: Acta Vet Hung Source Type: research

Detection and first molecular characterisation of three picornaviruses from diarrhoeic calves in Turkey.
n B Abstract The involvement of picornaviruses in calf diarrhoea was evaluated by the analysis of 127 faecal samples collected from diarrhoeic calves during 2014-2016. Virus detections were carried out by PCR using generic or specific primer pairs. One-third of the faecal samples (33.86%) were found to be positive for one or more of the studied viruses. Bovine kobuvirus was detected in 22.83%, bovine hungarovirus in 11.02%, while bovine enterovirus 1 in 5.51% of the samples. The sequences of the PCR products indicated the existence of novel variants in all the three virus species. When comparing the partial sequen...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Işidan H, Turan T, Atasoy MO, Sözdutmaz I, Irehan B Tags: Acta Vet Hung Source Type: research

The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.
ron;ević M Abstract The greatest epidemiological significance of leptospirosis in Europe comes from the fact that it is the most widespread zoonosis in the world. However, epizootiological data, especially information on maintenance hosts such as small wild mammals, are largely missing. To fill this gap in data in Serbia, we used RT-PCR for the detection of pathogenic Leptospira species and analysed 107 animals belonging to six species of small wild mammals (Apodemus agrarius, Apodemus flavicollis, Microtus arvalis, Myodes glareolus, Microtus subterraneus and Sorex araneus) collected from two localities. The anim...
Source: Acta Veterinaria Hungarica - September 1, 2019 Category: Veterinary Research Authors: Blagojević J, Šekler M, Rajičić M, Pejić B, Budinski I, Jovanović VM, Adnađević T, Vidanović D, Matović K, Vujošević M Tags: Acta Vet Hung Source Type: research

Relationships among some serum enzymes, negative energy balance parameters, parity and postparturient clinical (endo)metritis in Holstein Friesian cows - Short communication.
olnár L, Kovács L, Szenci O Abstract Activities of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase, and concentrations of serum metabolites [beta-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFA)] of primiparous (n = 83) and multiparous (n = 213) Holstein cows were studied as possible predictors of retained fetal membranes (RFM), grade 2 clinical metritis (CM) and clinical endometritis (CEM). A logistic regression model was used to calculate odds ratios (OR) for the prevalence of CM diagnosed between 0-5, 6-10 and 11-20 days in milk (DIM) and for the prevalence...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Andela BG, Van Eerdenburg FJCM, Choukeir A, Buják D, Szelényi Z, Boldizsár S, Kézér FL, Molnár L, Kovács L, Szenci O Tags: Acta Vet Hung Source Type: research

Cryopreservation of gander semen in cryovials - Comparative study.
B, Liptói K, Kiss C, Barna J Abstract The aim of the study was to find a practical and inexpensive method for freezing goose semen for use in routine inseminations under farm conditions. Two basic freezing protocols [(1) dynamic, programmable freezing and (2) static, nitrogen vapour method] were evaluated with varying concentrations of dimethylformamide (DMF) plus additional osmoprotectants such as betaine, trehalose, and sucrose, using cryovials as containers. Altogether eight different treatments were compared. sperm viability before freezing and after thawing was examined by in vitro tests and, in the ca...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Váradi É, Drobnyák Á, Végi B, Liptói K, Kiss C, Barna J Tags: Acta Vet Hung Source Type: research

Effect of single-nucleotide polymorphisms on specific reproduction parameters in Hungarian Large White sows.
y J, Zsolnai A, Anton I Abstract The aim of this study was to reveal the effect of single-nucleotide polymorphisms (SNPs) on the total number of piglets born (TNB), the litter weight born alive (LWA), the number of piglets born dead (NBD), the average litter weight on the 21st day (M21D) and the interval between litters (IBL). Genotypes were determined on a high-density Illumina Porcine SNP 60K BeadChip. Data screening and data identification were performed by a multi-locus mixed-model. Statistical analyses were carried out to find associations between individual genotypes of 290 Hungarian Large White sows and the...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Balogh EE, Gábor G, Bodó S, Rózsa L, Rátky J, Zsolnai A, Anton I Tags: Acta Vet Hung Source Type: research

Reference values for fetal heart rate in cattle in the first trimester of pregnancy.
ute;r L, Szenci O Abstract The aim of this study was to create a fetal heart rate (FHR) reference curve for singleton bovine fetuses in the first trimester of gestation and to determine its possible relationship with the outcome of pregnancy. Forty-eight Holstein-Friesian cows with one fetus and five cows with twins were used. Fetal heart beatings were recorded on videotape during transrectal scanning with a 5 and/or 7.5 MHz linear array transducer on a weekly basis between Days 40 and 95 of gestation. FHR was calculated by averaging the results of five counts of the same record by the same observer. For singleton...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Lénárt L, Taverne M, Wolleswinkel P, Gubik Z, Molnár L, Szenci O Tags: Acta Vet Hung Source Type: research

Merle allele variations in the Mudi dog breed and their effects on phenotypes.
rt X, Ninausz N, Varga L, Zenke P Abstract A retrotransposon insertion in the SILV gene is associated with a peculiar phenotype of dog, known as a merle. It is characterised by various areas of their coat colour becoming diluted due to a malfunction in the eumelanin-producing pigment cells. Recent studies have shown that the exact size of the short interspersed element (SINE) insertion is in correlation with specific phenotypic attributes, but was not able to absolutely confine dogs to a certain colour pattern. Our study focused on the merle variations occurring in the Mudi breed. Altogether, 123 dog samples from ...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Pelles Z, Gáspárdy A, Zöldág L, Lénárt X, Ninausz N, Varga L, Zenke P Tags: Acta Vet Hung Source Type: research

Anaesthetic management and complications of canine adrenalectomies: 41 cases (2007-2017).
This study highlights some possible association between the anaesthetic management and the outcome of the procedure. PMID: 31238728 [PubMed - in process] (Source: Acta Veterinaria Hungarica)
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Merlin T, Veres-Nyéki K Tags: Acta Vet Hung Source Type: research

No polymorphisms in the coding region of the prion-like protein gene in Thoroughbred racehorses.
Abstract Prion diseases are fatal neurodegenerative diseases characterised by the accumulation of an abnormal prion protein isoform (PrPSc), which is converted from the normal prion protein (PrPC). Prion diseases have been reported in an extensive number of species but not in horses up to now; therefore, horses are known to be a species resistant to prion diseases. The prion-like protein gene (PRND) is closely located downstream of the prion protein gene (PRNP) and the prion-like protein (Doppel) is a homologue with PrP. Previous studies have shown that an association between prion diseases and polymorphisms of th...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Jeong MJ, Jeong BH Tags: Acta Vet Hung Source Type: research

Production and evaluation of leukocyte- and thrombocyte-rich fibrin membranes in birds.
In conclusion, the centrifugation protocol allowed the production of L-TRF membranes in the four bird species studied. Histologically, cell ratios were analogous in domestic chickens and parrots, and macaws had the highest ratio of thrombocytes. PMID: 31238730 [PubMed - in process] (Source: Acta Veterinaria Hungarica)
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Fernandes LL, Rahal SC, Fabro AT, Batah SS, Hippólito AG, Bisca JM, Hirot IN, Teixeira CR Tags: Acta Vet Hung Source Type: research

Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs.
cute;lint Á, Oláh I Abstract The aim of this immunocytochemical study was to compare mannose-binding lectin (MBL) production induced by avian coronavirus in the spleen and caecal tonsil (CT). One-day-old specific-pathogen-free (SPF) chickens were experimentally infected with six QX field isolates and the H120 vaccine strain. In the negative control birds, the spleen was MBL negative, while the CT showed scattered MBL-positive cells in close proximity and within the surface epithelium and germinal centre (GC)-like cell clusters. MBL was detectable in the ellipsoid-associated cells (EACs) and cell clus...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Farsang A, Bódi I, Fölker O, Minkó K, Benyeda Z, Bálint Á, Oláh I Tags: Acta Vet Hung Source Type: research

Isolation and characterisation of porcine epidemic diarrhoea virus in Hungary - Short communication.
This study was extended with a variety of samples collected from three separate farms with newly identified PEDV in 2018. The recombinant PEDV from 2016 was isolated successfully along with three viruses from 2018, and one isolate from the new cases was used for whole genome determination. Whole genome sequence alignment revealed the highest identity with recombinant Hungarian and Slovenian PEDV within the low-pathogenic European viruses. This suggests that these recombinant PEDV are circulating in this area and may spread to other parts of the continent. PMID: 31238732 [PubMed - in process] (Source: Acta Veterinaria Hungarica)
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Valkó A, Albert E, Cságola A, Varga T, Kiss K, Farkas R, Rónai Z, Biksi I, Dán Á Tags: Acta Vet Hung Source Type: research

Seroprevalence of Anaplasma phagocytophilum, Ehrlichia spp. and Borrelia burgdorferi infections in horses: first report from Northern Bulgaria - Short communication.
Abstract Lyme borreliosis, granulocytic anaplasmosis and monocytic ehrlichiosis are well studied in humans and dogs. In horses, these diseases are not widely investigated and limited information is available about their occurrence. The purpose of this study was to present the first ELISA-based report on the seroprevalence of Anaplasma phagocytophilum, Ehrlichia spp. and Borrelia burgdorferi in horses from Northern Bulgaria. A total of 192 horses were investigated from three regions in Northern Bulgaria (Northwestern, North-Central and Northeastern Bulgaria). All equine sera were tested for A. phagocytophilum, Ehrl...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Tsachev I, Baymakova M, Pantchev N Tags: Acta Vet Hung Source Type: research

Toxoplasma gondii genotypes circulating in domestic pigs in Serbia.
In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bio-assays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6, alt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). I...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Kuruca L, Uzelac A, Klun I, Lalošević V, Djurković-Djaković O Tags: Acta Vet Hung Source Type: research

Distinctive site preference of the fish parasite Myxobolus cerebralis (Cnidaria, Myxozoa) during host invasion.
, Herczeg D Abstract Here, we experimentally studied the site preference of Myxobolus cerebralis, one of the most pathogenic myxozoan (Cnidaria, Myxozoa) fish parasites, which causes whirling disease in salmonids. Parasite invasion was examined in three fish species with various susceptibility levels: the type host brown trout, the highly susceptible rainbow trout, and the non-susceptible gibel carp, in which parasite spores do not develop. We investigated the first two hours of fish invasion, and measured the site preference of triactinomyxons (TAMs) during attachment and penetration of fish in three body parts (...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Eszterbauer E, Sipos D, Szakály Á, Herczeg D Tags: Acta Vet Hung Source Type: research

Epidemiology, clinical and cytological features of lymphoma in Boxer dogs.
Abstract The aim of this study was to evaluate the epidemiology, clinical and laboratory characteristics of canine lymphomas as well as some aspects of treatment outcomes. The study was conducted on Boxer dogs with lymphoma diagnosed by cytology and immunocytochemistry (CD3 and CD79 alpha). During the study period, lymphoma was diagnosed in 63 Boxers; 86.8% were T-cell (based on the Kiel classification: small clear cell lymphoma, pleomorphic small cell lymphoma, pleomorphic mixed T-cell lymphoma, pleomorphic large T-cell lymphoma, lymphoblastic lymphoma/acute lymphoblastic leukaemia) and 13.2% were B-cell lymphoma...
Source: Acta Veterinaria Hungarica - June 1, 2019 Category: Veterinary Research Authors: Jankowska U, Jagielski D, Czopowicz M, Sapierzyński R Tags: Acta Vet Hung Source Type: research

Cardiopulmonary parasitic nematodes of the red fox (Vulpes vulpes) in Serbia.
ić I Abstract Among the wild canids, the red fox (Vulpes vulpes) is recognised as an important reservoir species for a range of parasites, including cardiopulmonary nematodes of public health and veterinary importance. As cross-host transmission between the red fox and domestic carnivores can play an important role in the epizootiology of cardiopulmonary parasitic diseases, the aim of the present investigations was to obtain data on the geographical distribution of cardiopulmonary nematodes of the red fox. The material for examination consisted of 83 foxes which were legally hunted at different locations during a...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Gavrilović P, Dobrosavljević I, Vasković N, Todorović I, Živulj A, Kureljušić B, Pavlović I Tags: Acta Vet Hung Source Type: research

First molecular detection of ball python nidovirus in Italy - Short communication.
Abstract A retrospective study was conducted to investigate the presence of ferlavirus, ball python nidovirus and bacteria in 32 tracheobronchial lavages from ball pythons raised in captivity and affected by respiratory disease. A touchdown reverse transcription polymerase reaction (RT-PCR) was performed to detect ball python nidovirus RNA targeting a 260-bp portion of the ORF1a gene, while a nested RT-PCR was applied to identify RNA targeting the 518-bp ferlavirus partial L gene. RT-PCR positive products were submitted for Sanger's sequencing and phylogeny reconstruction. Bacteriological examinations were perform...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Rampacci E, Masi M, Origgi FC, Stefanetti V, Bottinelli M, Selleri P, Coletti M, Passamonti F Tags: Acta Vet Hung Source Type: research

Measurement of the femoral neck angle in medium and large dog breeds using computed tomography.
Abstract The aim of this study was to get precise normal values of the femoral neck angle (FNA) in support of developing an optimally functioning total hip prosthesis for medium and large dog breeds. Accordingly, two- and three-dimensional computed tomographic images of the anatomical structures of the proximal femora of 58, hip-dysplasia-free, mature dogs of medium and large breeds were studied. Based on the length of their femora the dogs were allocated to Group I (from 145 to 195 mm) and Group II (from 196 to 240 mm). The FNA was measured on each femur using multi-slice spiral computed tomography (CT). The two-...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Al Aiyan A, Richardson K, Manchi G, Plendl J, Brunnberg L Tags: Acta Vet Hung Source Type: research

First identification of porcine parvovirus 3 in a wild boar in Italy by viral metagenomics - Short communication.
Abstract Metagenomic analysis revealed the presence of porcine parvovirus 3 (PPV3) in the pool of the internal organs of a wild boar found dead in Southern Italy. Phylogenetic analysis based on the complete coding sequences showed that the newly detected virus is most closely related to those found also in wild boars in Romania during 2010-2011. Even though the death could not be associated with this virus, PPV3 could have contributed to lowering the host's immunological defences. PMID: 30922086 [PubMed - in process] (Source: Acta Veterinaria Hungarica)
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Amoroso MG, Cerutti F, D'Alessio N, Lucibelli MG, Cerrone A, Acutis PL, Galiero G, Fusco G, Peletto S Tags: Acta Vet Hung Source Type: research

Trichinella spp. in wild mesocarnivores in an endemic setting.
Abstract Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Klun I, Ćosić N, Ćirović D, Vasilev D, Teodorović V, Djurković-Djaković O Tags: Acta Vet Hung Source Type: research

Frequency of diarrhoea-associated viruses in swine of various ages in Hungary.
te;nai Z, Dán Á Abstract Enteric viral diseases of swine are one of the most frequent disorders causing huge economic losses in pork production. After the reappearance of an emerging enteropathogen, porcine epidemic diarrhoea virus (PEDV) in Hungary in 2016, an extensive survey was initiated in an attempt to identify diarrhoea-related porcine viruses, including adeno-, astro-, boca-, calici-, circo-, corona-, kobu-, rota- and Torque teno viruses. A total of 384 faecal samples collected during a twoyear period from diarrhoeic and asymptomatic pigs of various ages in 17 farms were screened by conventio...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Valkó A, Marosi A, Cságola A, Farkas R, Rónai Z, Dán Á Tags: Acta Vet Hung Source Type: research

Epidemiology of human trichinellosis in Vojvodina province, Serbia, from 2005 to 2016.
ić S Abstract Trichinellosis is one of the most important foodborne diseases in the Eastern European countries. The main objective of this study was to investigate the epidemiological patterns of trichinellosis outbreaks that occurred between 2005 and 2016 in Vojvodina, a northern province of the Republic of Serbia. The average incidence was 3.5 per 100,000 inhabitants. A total of 828 people acquired the infection. The disease occurred in all age groups, slightly more often in males, and quite frequently in a severe form considering the high share of hospitalised patients and the fatal outcome rate (41.6 ±...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Petrović J, Grgić Ž, Prodanov Radulović J, Ratajac R, Urošević M, Pustahija T, Medić S Tags: Acta Vet Hung Source Type: research

First molecular detection of Apis mellifera filamentous virus in honey bees (Apis mellifera) in Hungary.
We examined 664 honey bee samples that had been collected during winter mortality from three seemingly healthy colonies and from one colony infested heavily by the parasitic mite Varroa destructor in 2016 and 2017. The subsequent PCR screening of honey bee samples revealed the abundant presence of Apis mellifera filamentous virus (AmFV) for the first time in Central Europe. Based on phylogeny reconstruction, the newly-detected virus was found to be most closely related to a Chinese AmFV strain. More sequence data from multiple countries would be needed for studying the detailed phylogeographical patterns and worldwide spre...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Zana B, Geiger L, Kepner A, Földes F, Urbán P, Herczeg R, Kemenesi G, Jakab F Tags: Acta Vet Hung Source Type: research

Prevalence and risk factors of Neospora caninum infection in ovine flocks of central-western Mexico.
This study, carried out in 13 sheep flocks of central-western Mexico, aimed to identify the prevalence of anti-N. caninum antibodies, to develop a risk analysis of the infection and to estimate the prevalence of parasite DNA in blood. A total of 368 serum and blood samples were subjected to ELISA and PCR tests, respectively, and the association between the prevalence of antibodies and some management factors was estimated. The overall prevalence of anti-N. caninum antibodies was 13.5% [50/368; 95% confidence interval (CI) 10-17], ranging from 2.7 to 90% per flock, and 92% of the farms had seropositive animals. In ewes the ...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Romo-Gallegos JM, Cruz-Vázquez C, Medina-Esparza L, Ramos-Parra M, Romero-Salas D Tags: Acta Vet Hung Source Type: research

First evidence of tick-borne protozoan pathogens, Babesia sp. and Hepatozoon canis, in red foxes (vulpes vulpes) in Serbia.
Abstract Tick-borne haematozoans cause severe diseases in domestic animals, and some of them have zoonotic potential. The results of previous studies in Europe point to the important role of foxes in natural endemic cycles of several tick-borne pathogens, including protozoa. The aim of the present research was to acquire information on the prevalence and distribution of tick-borne protozoan parasites among foxes in Serbia. Legally hunted foxes from 14 localities throughout Serbia were analysed. Spleen samples were collected from 129 animals and tested for the presence of Babesia spp. and Hepatozoon spp. by PCR. In...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Juwaid S, Sukara R, Penezić A, Mihaljica D, Veinović G, Kavallieratos NG, Ćirović D, Tomanović S Tags: Acta Vet Hung Source Type: research

Immunopathological findings in a cat with auricular chondritis.
e Castro MB Abstract At clinical examination, a 5-year-old male domestic short-haired cat exhibited painful swelling and erythema of the pinnae of both ears. Microscopically, the lesions on both pinnae were composed of diffuse granulomatous chondritis with degeneration and necrosis of the pinnal cartilage. Numerous mast cells were also observed within and surrounding the inflammatory lesion. Immunohistochemistry showed a mixed inflammatory infiltrate characterised by the predominance of macrophages (CD68+, MAC 387+ and Lysozyme+), T lymphocytes (CD3+), some B lymphocytes (CD79α+) and neutrophils. Immunopatho...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Meziara Wilson T, Machado M, De Sousa DER, Braúna T, Torres Neto R, Laufer-Amorim R, Szabó MPJ, De Castro MB Tags: Acta Vet Hung Source Type: research

Changes in novel gastrointestinal and renal injury markers in the blood plasma of sheep following increasing intravenous doses of tolfenamic acid.
This study was aimed at determining the changes in gastrointestinal (TFF2 and HYP), renal (NGAL and KIM-1) and cardiac (cTn-I, CK-MB) injury markers after the use of increasing intravenous doses of TA in sheep. TA was administered intravenously to groups of six sheep each, at the dose levels of 0 (Group 0, i.e., G0), 2 (G2), 4 (G4), 8 (G8) and 16 (G16) mg/kg. The concentrations of the studied biomarkers were measured at 3, 9, 18 and 36 h after administration of TA. The TFF2 and NGAL concentrations in G16 were found to be significantly higher (P
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Yildiz R, Corum O, Atik O, Durna Corum D, Altan F, Ok M, Uney K Tags: Acta Vet Hung Source Type: research

Identification of neuropeptide y in superior cervical ganglion neurons that project to the oesophagus - A combined immunohistochemical labelling and retrograde tracing study in pigs.
This study used retrograde neuronal tracing and immunofluorescence methods to analyse NPY-positive neurons located in superior cervical ganglion and supplying the cervical oesophagus in the pig. The presence of NPY was observed in 30% of all neurons supplying the part of oesophagus studied. Probably the number of Fast Blue (FB) positive cells depends on the area of the wall injected with FB and the fragment of oesophagus studied. Therefore, the obtained results indicate that the described peptide is an important factor in the extrinsic innervation of this part of the GI tract. PMID: 30922095 [PubMed - in process] (Sou...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Rytel L, Snarska A, Gonkowski S, Wojtkiewicz J, Szenci O, Sobiech P Tags: Acta Vet Hung Source Type: research

Genotyping of four loci in Hungarian Yellow and broiler chickens.
pp Á Abstract Traditional selection has led to remarkable differences in allele frequencies among various chicken breeds. Indigenous and broiler-type chicken populations were genotyped for polymorphisms in thyroid hormone responsive Spot14α, prolactin (PRL), IGF-binding protein 2 (IGFBP2), and somatostatin (SST) genes in order to determine potential utilisation type-associated allele frequencies. Significant (P
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Szalai K, Tempfli K, Lencsés-Varga E, Bali Papp Á Tags: Acta Vet Hung Source Type: research

Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.
Abstract Tris (hydroxymethyl) aminomethane (Tris) has been used as a pH regulator for buffering the pH of dilution extenders for boar semen, such as the Modena extender. The purpose of the present study was to assess the effects of Tris supplementation at different concentrations (0, 8, 24 and 72 μM) into the freezing extender on the quality and fertilising capacity of frozen-thawed boar spermatozoa. The results showed that the supplementation of 24 μM of Tris gave significantly higher percentages of sperm viability and plasma membrane integrity than those of the control group at any time point of assessment...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Namula Z, Tanihara F, Wittayarat M, Hirata M, Nguyen NT, Hirano T, Le QA, Nii M, Otoi T Tags: Acta Vet Hung Source Type: research

Assessment of serum allergen-specific IgE levels in horses with seasonal allergic dermatitis and recurrent airway obstruction in Spain.
dez A Abstract Allergic conditions are prevalent equine diseases that can be diagnosed by clinical examination alone, but definitive diagnosis is more likely with laboratory testing. The ELISA Allercept© test was used to analyse the serum samples of 73 horses with allergic diseases. Sixty-one horses (83.5%) had allergen-specific IgE levels ≥ 150 ELISA Units (EU), the cut-off defined by the assay. Fifty-four horses had allergic dermatitis (AD) with high IgE levels to Tyrophagus putrescentiae (51.9%), Rumex crispus (48.1%), Tabanus (46.3%) and Dermatophagoides farinae/ D. pteronyssinus (40.7%). Seven horses ...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Marteles D, Odriozola L, Verde MT, Conde T, Fernández A Tags: Acta Vet Hung Source Type: research

Pregnancy and stillbirth losses in dairy cows with singleton and twin pregnancies.
ute;si A, Molnár L, Szenci O Abstract The aim of this study was to examine the effect of twin pregnancy, fetal laterality, the number of corpora lutea (CL) and cavitary CL on pregnancy losses in Holstein-Friesian cows with a positive pregnancy diagnosis based on ultrasonography between days 29-42 after AI. Pregnancy was confirmed by transrectal palpation between days 57-70 after AI and at the time of drying-off as well. Twin pregnancy rate was 8.4% at the time of the early pregnancy examination. Pregnancy loss did not differ between singleton- and twin-carrying animals either between days 57-70 of gestation...
Source: Acta Veterinaria Hungarica - March 1, 2019 Category: Veterinary Research Authors: Szelényi Z, Győri D, Boldizsár S, Kovács L, Répási A, Molnár L, Szenci O Tags: Acta Vet Hung Source Type: research

Calculation of the required transfusion volume in anaemic Holstein calves.
, Marcus I Abstract In bovine medicine, blood transfusion practice represents an emergency therapy where time is critical. The aim of this study was to develop a mathematical model to calculate the required blood for transfusion, using parameters which could be easily determined on the spot. Twenty Holstein calves were assigned to two groups depending on body weight (100.1 ± 3.1 kg and 151.1 ± 3.0 kg, respectively). All animals were subjected to an anaemia induction protocol in isovolaemic conditions in order to reduce the packed cell volume (PCV) by more than one third. Twenty-four hours later, each...
Source: Acta Veterinaria Hungarica - December 1, 2018 Category: Veterinary Research Authors: Muntean VI, Sárpataki O, Potârniche AV, Meghzili H, Sevastre B, Marcus I Tags: Acta Vet Hung Source Type: research

Sinonasal cysts causing dyspnoea in two cattle - case report.
Abstract Two cattle were referred to the University Clinic for Ruminants of the University of Veterinary Medicine in Vienna. The main clinical sign in both cattle was dyspnoea with nasal stridor. Clinical examination of the upper respiratory tract was conducted, supplemented by ultrasonography, endoscopy and radiography. In addition, histological, bacteriological, and cytological examinations of different specimen materials were performed. The cow of Case 1 suffered from cystic nasal conchae, which was treated successfully by a laser technique. The cow of Case 2 also suffered from cystic nasal conchae. No surgery ...
Source: Acta Veterinaria Hungarica - December 1, 2018 Category: Veterinary Research Authors: Stanitznig A, Velde K, Wittek T, Franz S Tags: Acta Vet Hung Source Type: research

The response of prion genic variation to selection for scrapie resistance in Hungarian indigenous sheep breeds.
ute;fár L, Bali Papp Á, Kovács E Abstract The authors studied the present status of Hungarian indigenous sheep breeds based on the genetic background of scrapie resistance. The aim of this investigation was to estimate the relative frequency of prion haplotypes, genotypes and risk categories, as well as to reveal the efficiency of the scrapie eradication programme achieved over the last decade. A novel approach in the characterisation of prion by using its genic variation was also implemented. The authors established that the proportion of deleterious sites (%) can be a useful indicator of the...
Source: Acta Veterinaria Hungarica - December 1, 2018 Category: Veterinary Research Authors: Gáspárdy A, Holly V, Zenke P, Maróti-Agóts Á, Sáfár L, Bali Papp Á, Kovács E Tags: Acta Vet Hung Source Type: research

Description of a new bat-associated bug species of the Cimex lectularius group from Vietnam.
J, Tan Tu V Abstract Cimex lectularius, the common bedbug is an important, emerging pest of both veterinary and medical importance. Here a recently discovered, genetically distinct new species of the C. lectularius group is described morphologically, as Cimex pulveratus Hornok sp. nov. PMID: 30580536 [PubMed - in process] (Source: Acta Veterinaria Hungarica)
Source: Acta Veterinaria Hungarica - December 1, 2018 Category: Veterinary Research Authors: Hornok S, Murányi D, Kontschán J, Tan Tu V Tags: Acta Vet Hung Source Type: research

A Holstein-Friesian dairy farm survey of postparturient factors influencing the days to first AI and days open in Hungary.
izsár S, Molnár L, Szenci O Abstract The authors monitored the postpartum period during the first seven weeks after calving at a Holstein-Friesian dairy farm in Hungary. Calvings occurred between 2013 and 2015 in three periods from June to November each year (n = 314). Data were collected from the farm record and ultrasonographic examinations were performed between 22 to 28 and 43 to 49 days in milk (DIM), respectively. The animals were followed until successful artificial insemination (AI), i.e. until becoming pregnant or until culling, but at most at 365 DIM. The prevalence of dystocia, twin calvin...
Source: Acta Veterinaria Hungarica - December 1, 2018 Category: Veterinary Research Authors: Buják D, Szelényi Z, Choukeir A, Kovács L, Kézér FL, Boldizsár S, Molnár L, Szenci O Tags: Acta Vet Hung Source Type: research