Expression, purification, crystallization and preliminary crystallographic analysis of a GH20 β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi
In this study, the crystallization and preliminary crystallographic data of wild-type VhGlcNAcase and its catalytically inactive mutant D437A in the absence and the presence of substrate are reported. Crystals of wild-type VhGlcNAcase were grown in 0.1 M sodium acetate pH 4.6, 1.4 M sodium malonate, while crystals of the D437A mutant were obtained in 0.1 M bis-tris pH 7.5, 0.1 M sodium acetate, 20% PEG 3350. X-ray data from the wild-type and the mutant crystals were collected at a synchrotron-radiation light source and were complete to a resolution of 2.5 Å. All crystals were composed of the same type of dimer, with the substrate N,N′-diacetylglucosamine (GlcNAc2 or diNAG) used for soaking was cleaved by the active enzyme, leaving only a single GlcNAc molecule bound to the protein.
Date: Thursday, 04 30, 2020; Speaker: Staff NIH Library; This class is presented as a webinar only; Videocast Event
Date: Monday, 03 30, 2020; Speaker: R. Burke Squires, Mr., NIAID / OCICB / BCBB; Webinar Only; Videocast Event
Date: Thursday, 04 02, 2020; Speaker: Staff NIH Library; This class is presented as a webinar only. ; Videocast Event
Date: Thursday, 04 09, 2020; Speaker: Zeynup Gumus, Assistant Professor, Ichan School of Medicine at Mount Sinai; https://cbiit.webex.com/mw3300/mywebex/default.do?
Date: Wednesday, 04 22, 2020; Speaker: Staff NIH Library; This class is presented as a webinar only. ; Videocast Event
A group of moderately ill people were given hydroxychloroquine, which appeared to ease their symptoms quickly, but more research is needed.
A member of the Arkansas House said Wednesday he's tested positive for the coronavirus and state health officials reported two more people have died from the outbreak.
Publication date: May 2020Source: Molecular Immunology, Volume 121Author(s): Lingmin Huang, Ruiheng Luo, Jing Li, Dan Wang, Yening Zhang, Liping Liu, Ningjie Zhang, Xueming Xu, Ben Lu, Kai Zhao
Publication date: Available online 1 April 2020Source: Autoimmunity ReviewsAuthor(s): Klaus Wirth, Carmen Scheibenbogen
Publication date: Available online 1 April 2020Source: Analytic Methods in Accident ResearchAuthor(s): Mouyid Islam, Fred Mannering