Growth and toxin production of phomopsin A and ochratoxin A forming fungi under different storage conditions in a pea (Pisum sativum) model system

AbstractPhomopsins are mycotoxins mainly infesting lupines, with phomopsin A (PHOA) being the main mycotoxin. PHOA is produced byDiaporthe toxica, formerly assigned as toxigenicPhomopsis leptostromiformis, causing infections in lupine plants and harvested seeds. However,Diaporthe species may also grow on other grain legumes, similar toAspergillus westerdijkiae as an especially potent ochratoxin A (OTA) producer. Formation of PHOA and OTA was investigated on whole field peas as model system to assess fungal growth and toxin production at adverse storage conditions. Field pea samples were inoculated with the two fungal strains at two water activity (aw) values of 0.94 and 0.98 and three different levels of 30, 50, and 80% relative air humidity.After 14  days at anaw value of 0.98, the fungi produced 4.49 to 34.3  mg/kg PHOA and 1.44 to 3.35 g/kg OTA, respectively. Strains ofD. toxica also tested showed higher PHOA concentrations of 28.3 to 32.4  mg/kg.D. toxica strains did not grow or produce PHOA at anaw values of 0.94, whileA. westerdijkiae still showed growth and OTA production.Elevated water activity has a major impact both on OTA and, even more pronouncedly, on PHOA formation and thus, proper drying and storage of lupins as well as other grain legumes is crucial for product safety.

Source: Mycotoxin Research - December 18, 2021 Category: Toxicology Source Type: research