Enantiomeric Separation in Comprehensive Two‐dimensional Gas Chromatography with Accurate Mass Analysis.

Enantiomeric Separation in Comprehensive Two‐dimensional Gas Chromatography with Accurate Mass Analysis.
Chirality. 2014 Jan 13;
Authors: Chin ST, Nolvachai Y, Marriott PJ
Abstract
Chiral comprehensive two‐dimensional gas chromatography (eGC×GC) coupled to quadrupole–accurate mass time‐of‐flight mass spectrometry (QTOFMS) was evaluated for its capability to report the chiral composition of several monoterpenes, namely, α‐pinene, β‐pinene, and limonene in cardamom oil. Enantiomers in a standard mixture were fully resolved by direct enantiomeric‐GC analysis with a 2,3‐di‐O‐methyl‐6‐t‐butylsilyl derivatized β‐cyclodextrin phase; however, the (+)‐(R)‐limonene enantiomer in cardamom oil was overlapped with other background components including cymene and cineole. Verification of (+)‐(R)‐limonene components based on characteristic ions at m/z 136, 121, and 107 acquired by chiral single‐dimension GC−QTOFMS in the alternate MS/MSMS mode of operation was unsuccessful due to similar parent/daughter ions generated by interfering or co‐eluting cymene and cineole. Column phases SUPELCOWAX, SLB‐IL111, HP‐88, and SLB‐IL59, were incorporated as the second dimension column ((2) D) in chiral GC×GC analysis; the SLB‐IL59 offered the best resolution for the tested monoterpene enantiomers from the matrix background. Enant...

Source: Chirality - January 13, 2014 Category: Molecular Biology Authors: Chin ST, Nolvachai Y, Marriott PJ Tags: Chirality Source Type: research