Sandwich-Based Antibody Arrays for Protein Detection.

Sandwich-Based Antibody Arrays for Protein Detection.
Methods Mol Biol. 2021;2237:1-10
Authors: Mao YQ, Petritis B, Huang RP
Abstract
Sandwich-based antibody arrays enable the detection of multiple proteins simultaneously, thus offering a time- and cost-effective alternative to single-plex platforms. The protein of interest is "sandwiched" between an antibody that captures it to the array and a second antibody that is used for detection. Here we describe a 1-day procedure to process samples, such as serum or cell lysates, with a quantitative sandwich-based antibody array on a glass substrate using fluorescence.
PMID: 33237404 [PubMed - as supplied by publisher]

Source: Mol Biol Cell - November 26, 2020 Category: Molecular Biology Authors: Mao YQ, Petritis B, Huang RP Tags: Methods Mol Biol Source Type: research