Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.

Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.
Methods Mol Biol. 2021;2236:203-217
Authors: Rambault M, Borkute R, Doz-Deblauwe E, Le-Vern Y, Winter N, Dorhoi A, Remot A
Abstract
Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
PMID: 33237550 [PubMed - as supplied by publisher]

Source: Mol Biol Cell - November 26, 2020 Category: Molecular Biology Authors: Rambault M, Borkute R, Doz-Deblauwe E, Le-Vern Y, Winter N, Dorhoi A, Remot A Tags: Methods Mol Biol Source Type: research