ATP-dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: expression, purification and crystallization

A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP-dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). Following expression, the protein was purified and crystallized using the vapor-diffusion method. The protein crystals were optimized at a 1:1 protein:reservoir solution ratio using PPGBA 2000. The optimized crystals diffracted to a dmin of 3.15   Å . The collected data revealed preliminary structural information regarding this newly discovered protein.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: Trypanosoma brucei ATP-dependent RNA helicases ZC3H41 helicase C domain protozoan proteins DEAD-box proteins research communications Source Type: research