HIPK2 sustains inflammatory cytokine production by promoting endoplasmic reticulum stress in macrophages.

HIPK2 sustains inflammatory cytokine production by promoting endoplasmic reticulum stress in macrophages.
Exp Ther Med. 2020 Dec;20(6):171
Authors: Xu L, Fang H, Xu D, Wang G
Abstract
Uncontrolled inflammatory cytokine production by macrophages contributes to numerous conditions, including infection, endotoxemia and sepsis. A previous study proposed that endoplasmic reticulum (ER) stress acts as an essential process in inflammatory cytokine production by macrophages. The present study used a mouse sepsis model and in vitro macrophages to demonstrate that homeodomain-interacting protein kinase 2 (HIPK2) sustained cytokine production in an ER stress-dependent manner. HIPK2 expression was upregulated in the early phase of lipopolysaccharide stimulation. HIPK2 knockdown attenuated IL-6 and TNF-α production, and p65 phosphorylation in macrophages. Furthermore, the attenuated cytokine production was abolished by the ER stress agonist tunicamycin. The activation of ER stress increased the levels of IL-6 and TNF-α, and the phosphorylation of p65, in macrophages following knockdown of HIPK2. Furthermore, HIPK2 inhibition attenuated the production of IL-6 and TNF-α in vitro and in vivo. Therefore, HIPK2 sustained inflammatory cytokine production by promoting ER stress in macrophages. Targeting HIPK2 may be a potential strategy for the management of uncontrolled inflammation in clinical settings.
PMID: 33101464 [PubMed]

Source: Experimental and Therapeutic Medicine - October 27, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research