Development of ADPribosyl ubiquitin analogs to study enzymes involved in Legionella infection.

In this study we use a modular approach for the synthesis of triazole-linked Ub ADPr and analogues thereof and determine their affinity towards the hydrolase DupA, and compare its proteolysis to natively linked Ub ADPr . The inhibitory effects of modified Ub on the canonical eukaryotic E1-enzyme Uba1 are investigated and rationalized in the context of a high-resolution crystal structure reported here. Finally, we show that the synthetic Ub ADPr can be used to effectively pull-down over-expressed DupA from cell lysate.
PMID: 33075184 [PubMed - as supplied by publisher]

Source: Chemistry - October 19, 2020 Category: Chemistry Authors: van der Heden van Noort G, Kim RQ, Misra M, Alexis G, Tomašković I, Shin D, Schindelin H, Filippov DV, Ovaa H, Dikic I Tags: Chemistry Source Type: research