Targeted next-generation sequencing and allele-specific qPCR of laser capture microdissected samples uncover molecular differences in mixed odontogenic tumors

The molecular pathogenesis of mixed odontogenic tumors has not been established and understanding their genetic basis could refine their classification and help define molecular markers for diagnostic purposes. We assessed potentially pathogenic mutations in the component tissues of 28 cases of mixed odontogenic tumors. Laser capture microdissected tissue from ten ameloblastic fibromas (AF), four ameloblastic fibrodentinomas (AFD), six ameloblastic fibro-odontomas (AFO), three ameloblastic fibrosarcomas (AFS), and five odontomas (OD) were screened by next-generation sequencing for a panel of 50 oncogenes and tumour suppressor genes and results confirmed by TaqMan allele-specific qPCR.

Source: Journal of Molecular Diagnostics - September 19, 2020 Category: Pathology Authors: Bruna Pizziolo Coura, Vanessa F átima Bernardes, Sílvia Ferreira de Sousa, Marina Gonçalves Diniz, Rennan Garcias Moreira, Bruno Augusto Benevenuto de Andrade, Mario José Romañach, Helder Antônio Rebelo Pontes, Ricardo Santiago Gomez, Edward William Source Type: research