In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.

In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.
Platelets. 2020 Sep 16;:1-8
Authors: Butov KR, Osipova EY, Mikhalkin NB, Trubina NM, Panteleev MA, Machlus KR
Abstract
Megakaryocytes (MKs) are relatively rare in bone marrow, comprising <0.05% of the nucleated cells, which makes direct isolation from human bone marrow impractical. As such, in vitro expansion of primary MKs from patient samples offers exciting fundamental and clinical opportunities. As most of the developed ex vivo methods require a substantial volume of biomaterial, they are not widely performed on young patients. Here we propose a simple, robust, and adapted method of primary human MK culture from 1 mL of bone marrow aspirate. Our technique uses a small volume of bone marrow per culture, uses straightforward isolation methods, and generates approximately 6 × 105 mature MKs per culture. The relative high cell purity and yield achieved by this technique, combined with efficient use of low volumes of bone marrow, make this approach suitable for diagnostic and basic research of human megakaryopoiesis.
PMID: 32936668 [PubMed - as supplied by publisher]

Source: Platelets - September 17, 2020 Category: Hematology Tags: Platelets Source Type: research