UiO-66-NH2 MOF-based ratiometric fluorescent probe for the detection of dopamine and reduced glutathione.

UiO-66-NH2 MOF-based ratiometric fluorescent probe for the detection of dopamine and reduced glutathione.
Talanta. 2020 Dec 01;220:121352
Authors: Wang N, Xie M, Wang M, Li Z, Su X
Abstract
Metal-organic framework materials (MOFs) are highly promising materials for biomedical applications owing to high porosity, adjustable pore structure and high loading capacity. In this paper, we herein reported a novel UiO-66-NH2 MOF-based ratiometric fluorescent probe for the high sensitive detection of dopamine and reduced glutathione. Light-emitting metal-organic framework materials UiO-66-NH2 MOF with a fluorescence emission wavelength of 450 nm was synthesized by a simple hydrothermal synthesis. Dopamine could self-oxidize in polyethyleneimine (PEI) solution to form copolymer (PDA-PEI), which can emit yellow-green fluorescence at 530 nm. PDA-PEI can quench the fluorescence of UiO-66-NH2 MOF via FRET and the fluorescence intensity of PDA-PEI at 530 nm is increasing. Due to the reductive properties of glutathione, the formation of PDA-PEI could be blocked and the fluorescence of the UiO-66-NH2 MOF could be restored. Therefore, dopamine and reduced glutathione could be detected simultaneously via monitoring the ratiometric fluorescence intensity (I530/I450). The ratiometric fluorescent method showed good linearity curve with the concentration of dopamine in the range of 4-50 μM and with the concentration of reduced glutathione in the range ...

Source: Talanta - September 17, 2020 Category: Chemistry Authors: Wang N, Xie M, Wang M, Li Z, Su X Tags: Talanta Source Type: research