Super-resolution spatial proximity detection with proximity-PAINT.
Super-resolution spatial proximity detection with proximity-PAINT.
Angew Chem Int Ed Engl. 2020 Sep 16;:
Authors: Schueder F, Lara-Gutiérrez J, Haas D, Beckwith KS, Yin P, Ellenberg J, Jungmann R
Abstract
Visualizing the functional interactions of biomolecules such as proteins and nucleic acids is key to understanding cellular life on the molecular scale. Spatial proximity is often used as a proxy for the direct interaction of biomolecules. However, current techniques to visualize spatial proximity are either limited by spatial resolution, dynamic range, or lack of single-molecule sensitivity. Here, we introduce Proximity-PAINT (pPAINT), a variation of the super-resolution microscopy technique DNA-PAINT. pPAINT uses a split-docking-site configuration to detect spatial proximity with high sensitivity, low false-positive rates, and tunable detection distances. We benchmark and optimize pPAINT using designer DNA nanostructures and demonstrate its cellular applicability by visualizing the spatial proximity of alpha- and beta-tubulin in microtubules using super-resolution detection.
PMID: 32936507 [PubMed - as supplied by publisher]
Source: Angewandte Chemie - Category: Chemistry Authors: Schueder F, Lara-Gutiérrez J, Haas D, Beckwith KS, Yin P, Ellenberg J, Jungmann R Tags: Angew Chem Int Ed Engl Source Type: research
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