In situ detection and imaging of lysophospholipids in zebrafish using matrix ‐assisted laser desorption/ionization fourier transform ion cyclotron resonance mass spectrometry

AbstractIn this paper, a matrix ‐assisted laser desorption/ionization fourier transform ion cyclotron resonance mass spectrometry (MALDI‐FTICR‐MS) imaging method was developed to rapid andin situ detect the spatial distribution of lysophospholipids (LPLs) in zebrafish. The combination of MALDI with ultrahigh ‐resolution FTICR‐MS achieves the MS imaging of LPLs with a mass resolution up to 50,000, which allows accurate identification and clear spatial visualization of LPLs in complex biological tissues. A series of lysophosphatidylcholines (LPCs) were detected using positive ion detection mode, and t heir concentration differences and spatial distributions were clearly visualized in different parts of zebrafish tissue. The method is rapid, simple, and efficient, being a desirable way to understand the spatial distribution of LPLs in biosome.

Source: Journal of Mass Spectrometry - July 29, 2020 Category: Chemistry Authors: Kaili He, Chao Chen, Jiewei Deng, Ya ‐Jun Hou, Zhangmin Xiang, Yunyun Yang Tags: SPECIAL ISSUE ‐ APPLICATION NOTE Source Type: research