Immunoaffinity Micro-Flow Liquid Chromatography/Tandem Mass Spectrometry for the Quantitation of PD1 and PD-L1 in Human Tumor Tissues.

CONCLUSIONS: A highly specific and sensitive immunoaffinity micro-flow LC/MS/MS method for the simultaneous quantification of PD1 and PD-L1 in tumor tissues was developed and implemented. This method combines the advantage of immuno-capture for analyte enrichment with the high specificity of detection of multiple surrogate peptides by LC/MS/MS. The quantification of PD1 and PD-L1 co-expression in tumor could help evaluate their role in assessing tumor type selection and patient stratification.
PMID: 32666620 [PubMed - as supplied by publisher]

Source: Rapid Communications in Mass Spectrometry : RCM - July 14, 2020 Category: Chemistry Authors: Zhu Y, Zalaznick J, Sleczka B, Parrish K, Yang Z, Olah T, Shipkova P Tags: Rapid Commun Mass Spectrom Source Type: research