GSE153451 The asynchronous establishment of chromatin 3D architecture between in vitro fertilized and uniparental preimplantation pig embryos [ChIP-seq]

Contributors : Feifei Li ; Danyang Wang ; Ruigao Song ; Chunwei Cao ; Zhihua Zhang ; Yu Wang ; Xiaoli Li ; Jiaojiao Huang ; Qiang Liu ; Naipeng Hou ; Bingxiang Xu ; Xiao Li ; Xiaomeng Gao ; Yan Jia ; Jianguo Zhao ; Yanfang WangSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Sus scrofaPigs are important animals for agricultural and biomedical research, and its assisted reproductive technologies are urgently needed to be improved. Determining the underlying mechanism of epigenetic reprogramming in the early stage of preimplantation embryos derived from in vitro fertilization (IVF), parthenogenesis (PA) and androgenesis (AG), will not only contribute to assisted reproduction technologies of pigs but also will shed light into early human development. We generated 3D chromatin profiles for pig somatic cells, IVF, PA and AG preimplantation embryos. We found that the chromosomes in the pig preimplantation embryos are enriched for superdomains, i.e., the larger than 10M compartment domains, which are much rarer in mice. Wheras, p(s) curves, compartments and TADs, are largely conserved in somatic cells, and are gradually establishing during preimplantation embryogenesis. In the uniparental pig embryos, the establishment of chromatin architecture was highly asynchronized at all levels from IVF embryos, and a remarkably strong decompartmentalization was observed during zygotic genome activation (ZGA). Finally, chromosomes originat...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Sus scrofa Source Type: research