Specific gyrA gene mutations correlate with high prevalence of discordant levofloxacin resistance in Mycobacterium tuberculosis isolates from Beijing, China

Although rapid, highly sensitive molecular diagnostics tests are useful for diagnosing fluoroquinolone (FQ)-resistant tuberculosis (TB), results of molecular testing versus conventional sequential phenotypic drug susceptibility testing (pDST) are frequently discordant. Here we determined the discordance rate of levofloxacin (LFX) resistance results obtained via MeltPro TB molecular testing versus pDST of clinical TB isolates collected in Beijing, China between January and December 2018. Isolates with discordant results were further subjected to LFX minimal inhibitory concentration (MIC) determinations and DNA sequence analysis to explore causes of discordance.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Regular Article Source Type: research