Mass Spectrometry-Based Determination of Thyroid Hormones and Their Metabolites in Endocrine Diagnostics and Biomedical Research – Implications for Human Serum Diagnostics

Exp Clin Endocrinol Diabetes 2020; 128: 358-374 DOI: 10.1055/a-1175-4610The wide spectrum of novel applications for the LC-MS/MS-based
analysis of thyroid hormone metabolites (THM) in blood samples and other
biological specimen highlights the perspectives of this novel technology.
However, thorough development of pre-analytical sample workup and careful
validation of both pre-analytics and LC-MS/MS analytics, is needed,
to allow for quantitative detection of the thyronome, which spans a broad
concentration range in these biological samples.This minireview summarizes recent developments in advancing
LC-MS/MS-based analytics and measurement of total concentrations of
THM in blood specimen of humans, methods in part further refined in the
context of previous achievements analyzing samples derived from cell-culture
or tissues. Challenges and solutions to tackle efficient pre-analytic sample
extraction and elimination of matrix interferences are compared. Options for
automatization of pre-analytic sample-preparation and comprehensive coverage
of the wide thyronome concentration range are presented. Conventional
immunoassay versus LC-MS/MS-based determination of total and free
THM concentrations are briefly compared. [...] © Georg Thieme Verlag KG Stuttgart · New YorkArticle in Thieme eJournals: Table o...

Source: Experimental and Clinical Endocrinology and Diabetes - June 15, 2020 Category: Endocrinology Authors: K öhrle, Josef Richards, Keith H. Tags: Mini-Review Source Type: research