Hypothermic Storage of Human Umbilical Vein Endothelial Cells and Their Hydrogel Constructs.

Hypothermic Storage of Human Umbilical Vein Endothelial Cells and Their Hydrogel Constructs. Biopreserv Biobank. 2020 Jun 09;: Authors: Zhang X, Cao Y, Zhao G Abstract Human umbilical vein endothelial cells (HUVECs) have great potential in tissue engineering, regenerative medicine, and clinical applications. There is an ever-increasing demand to provide living HUVECs and HUVECs-hydrogel constructs to end users when needed in cell-based therapy and clinical applications. However, current methods to provide living cells and their constructs are mainly continuous culture and cryopreservation, which are high-cost, labor-intensive, time-consuming and inflexible. The research about hypothermic storage of HUVECs and their hydrogel constructs is still limited. Here, we studied the cell survival of HUVECs without encapsulation (W/O Encap) or with encapsulation (alginate, alginate with carboxymethyl chitosan [CMCH]) at 4°C and 25°C during 7 days, respectively. Also, we explored the optimal CMCH concentration for hypothermic storage, which is 0.5% (w/v) at 4°C and 25°C. Moreover, we evaluated the cell attachment after hypothermic storage. Our results enable the hypothermic storage of HUVECs and HUVEC-hydrogel constructs, and facilitate their application in tissue engineering and clinical medicine. PMID: 32522021 [PubMed - as supplied by publisher]
Source: Biopreservation and Biobanking - Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research