A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat.

A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat.
Theor Appl Genet. 2020 Jun 07;:
Authors: Rani K, Raghu BR, Jha SK, Agarwal P, Mallick N, Niranjana M, Sharma JB, Singh AK, Sharma NK, Rajkumar S, Tomar SMS, Vinod
Abstract
KEY MESSAGE: A novel leaf rust resistance gene, LrM, introgressed from Aegilops markgrafii and mapped on chromosome 2AS using SSR- and SNP-based PCR markers will aid in broadening the genetic base of rust resistance in wheat. A new leaf rust resistance gene tentatively named LrM was introgressed from the diploid non-progenitor species Ae. markgrafii (2n = 2x = 14, genome CC) into common wheat using the nulli-5B mechanism. The introgression line ER9-700 showed a high degree of resistance against a wide spectrum of Puccinia triticina pathotypes. Genetic analysis was performed using the F1, F2, F2:3 and BC1F1 generations derived from the cross ER9-700/Agra Local. The results showed a single dominant gene for leaf rust resistance. The resistance gene LrM was mapped on chromosome arm 2AS using SSR- and SNP-based PCR markers. Preliminary mapping with SSR markers in the F2:3 population from the cross ER9-700/Agra Local identified two SSR markers flanking the LrM. SNPs were identified in the genomic region flanked by SSR markers, and SNP-based PCR markers were developed to construct the final map. Three SNP-based PCR markers co-segregated and mapped closest ...

Source: TAG. Theoretical and Applied Genetics - June 6, 2020 Category: Genetics & Stem Cells Authors: Rani K, Raghu BR, Jha SK, Agarwal P, Mallick N, Niranjana M, Sharma JB, Singh AK, Sharma NK, Rajkumar S, Tomar SMS, Vinod Tags: Theor Appl Genet Source Type: research