A high-throughput method for dereplication and assessment of metabolite distribution in Salvia species using LC-MS/MS.

This study proposes a solution to this problem by the optimization of chromatographic separation and mass spectrometry parameters. We report herein a direct and high-throughput strategy for natural product dereplication in five Salvia species using high-resolution ESI-QTOF-MS/MS data. In the present study, we were able to identify a total of forty-seven natural products in crude extracts of five Salvia species using MS/MS fragmentation data. In addition to dereplication of Salvia species, quantitative profiling of twenty-one bioactive constituents of the genus was also performed on an ion trap mass spectrometer. For the quantitation study, method development focused on chromatographic optimizations to achieve maximum sensitivity. The developed dereplication and quantitation strategy can be extended to develop comprehensive metabolic profiles of other plant genera and species and thus can prove useful in the field of drug discovery from plants.
PMID: 32211205 [PubMed]

Source: Adv Data - March 28, 2020 Category: Epidemiology Authors: Ul Haq F, Ali A, Akhtar N, Aziz N, Khan MN, Ahmad M, Musharraf SG Tags: J Adv Res Source Type: research