Simultaneous monitoring of the bioconversion from lysine to glutaric acidby ethyl chloroformate derivatization and gas chromatography-mass spectrometry.

Simultaneous monitoring of the bioconversion from lysine to glutaric acidby ethyl chloroformate derivatization and gas chromatography-mass spectrometry.
Anal Biochem. 2020 Mar 16;:113688
Authors: Han YH, Choi TR, Park YL, Song HS, Choi YK, Kim HJ, Bhatia SK, Gurav R, Park K, Park SH, Kim W, Yang YH
Abstract
Glutaric acid is a precursor of a plasticizer that can be used for the production of polyester amides, ester plasticizer, corrosion inhibitor, and others. Glutaric acid can be produced either via bioconversion or chemical synthesis, and some metabolites and intermediates are produced during the reaction. To ensure reaction efficiency, the substrates, intermediates, and products, especially in the bioconversion system, should be closely monitored. Until now, high performance liquid chromatography (HPLC) has generally been used to analyze the glutaric acid-related metabolites, although it demands separate time-consuming derivatization and non-derivatization analyses. To substitute for this unreasonable analytical method, we applied herein a gas chromatography - mass spectrometry (GC-MS) method with ethyl chloroformate (ECF) derivatization to simultaneously monitor the major metabolites. We determined the suitability of GC-MS analysis using defined concentrations of six metabolites (L-lysine, cadaverine, 5-aminovaleric acid, 2-oxoglutaric acid, glutamate, and glutaric acid) and their mass chromatograms, regression equations, regressi...

Source: Analytical Biochemistry - March 15, 2020 Category: Biochemistry Authors: Han YH, Choi TR, Park YL, Song HS, Choi YK, Kim HJ, Bhatia SK, Gurav R, Park K, Park SH, Kim W, Yang YH Tags: Anal Biochem Source Type: research