A multifaceted investigation on the effect of vacuum on the headspace solid-phase microextraction of extra-virgin olive oil.

A multifaceted investigation on the effect of vacuum on the headspace solid-phase microextraction of extra-virgin olive oil.
Anal Chim Acta. 2020 Mar 22;1103:106-114
Authors: Mascrez S, Psillakis E, Purcaro G
Abstract
Headspace solid-phase microextraction (HS-SPME) is an easy, effective, and selective technique for the extraction of volatiles and semi-volatiles compounds. For the latter, longer equilibration times are needed, which are typically shortened by applying agitation or heating the sample. A less explored way to improve the extraction kinetics of analytes with a low-affinity for the headspace is to sample under vacuum conditions. The methodology that evolved from this approach was termed "vacuum-assisted HS-SPME" (Vac-HS-SPME) and was mainly used for water- and solid-based samples. The aim of this work was to investigate the effect of vacuum when dealing with non-aqueous liquid samples. For this purpose, the volatile profile of extra virgin olive oil was analyzed using a divinylbenzene/carboxen/polydimethylsiloxane fiber followed by gas chromatography-mass spectrometry. The effects of extraction temperature and sampling time were investigated using traditional one-variable at a time approach and a two-variable central component design for both Vac-HS-SPME and regular HS-SPME. The results showed an important enhancement in the extraction of semi-volatile compounds when using Vac-HS-SPME, and improved the information gained f...

Source: Analytica Chimica Acta - February 23, 2020 Category: Chemistry Authors: Mascrez S, Psillakis E, Purcaro G Tags: Anal Chim Acta Source Type: research