Smartphone-assisted robust enzymes@MOFs-based paper biosensor for point-of-care detection

Publication date: Available online 20 February 2020Source: Biosensors and BioelectronicsAuthor(s): Xiaoxue Kou, Linjing Tong, Yujian Shen, Wangshu Zhu, Li Yin, Siming Huang, Fang Zhu, Guosheng Chen, Gangfeng OuyangAbstract:Portable devices featured with fast analysis and affordable methodologies for clinical diagnostics have stimulated the rapid development of point-of-care (POC) technologies, potentially lowering the mortality rate. Herein, we demonstrated a portable, robust, and user-friendly intelligent metal-organic frameworks (MOFs) paper device, called smartphone-assisted biomimetic MOFs nanoreactor colorimetric paper (SBMCP), for on-demand POC detection of endogenous biomolecules. The concept of this paper platform was analogous to the intracellular cascades signal transduction, wherein the single/multiple enzymes components trapped within a ZIF-8 exoskeleton allowed the sensitive and selective recognition of target analyte via the accessible micropores network of ZIF-8, and then transferred the recognition event to a visual color signal based on the cascade reaction. Meanwhile, the ZIF-8 exoskeleton also endowed the enzymes with significantly elevated stability. As a result, this robust and portable SBMCP sensor enabled to on-site analyze different important disease-related biomolecules through modulating the enzyme cascades, combining with a custom-designed smartphone for signal readout. In the SBMCP assay, no sophisticated instruments or professional skill of the us...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research