Standalone operation of an EGOFET for ultra-sensitive detection of HIV

Publication date: Available online 19 February 2020Source: Biosensors and BioelectronicsAuthor(s): Sunil Kumar Sailapu, Eleonora Macchia, Irene Merino-Jimenez, Juan Pablo Esquivel, Lucia Sarcina, G. Scamarcio, Shelley D. Minteer, Luisa Torsi, Neus SabatéAbstractA point-of-care (POC) device to enable de-centralized diagnostics can effectively reduce the time to treatment, especially in case of infectious diseases. However, many of the POC solutions presented so far do not comply with the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment free, and deliverable to users) guidelines that are needed to ensure their on-field deployment. Herein, we present the proof of concept of a self-powered platform that operates using the analysed fluid, mimicking a blood sample, for early stage detection of HIV-1 infection. The platform contains a smart interfacing circuit to operate an ultra-sensitive electrolyte-gated field-effect transistor (EGOFET) as a sensor and facilitates an easy and affordable readout mechanism. The sensor transduces the bio-recognition event taking place at the gate electrode functionalized with the antibody against the HIV-1 p24 capsid protein, while it is powered via paper-based biofuel cell (BFC) that extracts the energy from the analysed sample itself. The self-powered platform is demonstrated to achieve detection of HIV-1 p24 antigens in fM range, suitable for early diagnosis. From these developments, a cost-effective digital P...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research