Prevalence and significance of the commonest phosphorylated motifs in the human proteome: a global analysis.

Prevalence and significance of the commonest phosphorylated motifs in the human proteome: a global analysis.
Cell Mol Life Sci. 2020 Feb 12;:
Authors: Cesaro L, Pinna LA
Abstract
Protein phosphorylation is the most frequent post-translational modification by which the properties of eukaryotic proteins can be reversibly modified. In humans, over 500 protein kinases generate a huge phosphoproteome including more than 200,000 individual phosphosites, a figure which is still continuously increasing. The in vivo selectivity of protein kinases is the outcome of a multifaceted and finely tuned process where numerous factors play an integrated role. To gain information about the actual contribution to this process of local features that reflect the interaction of the protein targets with the catalytic site of the kinases, the prevalence of the commonest motifs determining the consensus sequence of Ser/Thr-specific kinases has been examined in the whole human phosphoproteome and in the phosphoproteomes generated by a panel of the 47 most pleiotropic protein kinases. Our analysis shows that: (1) most phosphosites do conform to at least one of the motifs considered, with a substantial proportion conforming to two or more of them; (2) some motifs, with special reference to the one recognized by protein kinase CK2 (pS/pT-x-x-E/D) are very promiscuous, being abundantly represented also at the phosphosites of all the other protein kinases considere...

Source: Cellular and Molecular Life Sciences : CMLS - February 11, 2020 Category: Cytology Authors: Cesaro L, Pinna LA Tags: Cell Mol Life Sci Source Type: research