Gamma subunit second transmembrane domain contributes to epithelial sodium channel gating and amiloride block.
Gamma subunit second transmembrane domain contributes to epithelial sodium channel gating and amiloride block. Am J Physiol Renal Physiol. 2013 Oct 9; Authors: Shi S, Kleyman TR Abstract The epithelial sodium channel (ENaC) is comprised of three homologous subunits. Channels composed solely of α and β subunits (αβ channels) exhibit a very high open probability (Po) and reduced sensitivity to amiloride, in contrast to channels composed of α and γ subunits or of all three subunits (i.e., αγ and αβγ channels). A mutant channel comprised of α and β subunits, and a chimeric γ subunit where the region immediately preceding (β12 and wrist) and encompassing the second transmembrane domain (TM2) was replaced with the corresponding region of the β subunit (γ-βTM2) displayed characteristics reminiscent of αβ channels, including a reduced potency of amiloride block and a loss of Na(+) self-inhibition (reflecting an increased Po). Substitutions at key pore-lining residues of the γ-βTM2 chimera enhanced the Na(+) self-inhibition response, whereas key γ subunit substitutions reduced the response. Furthermore, multiple sites within the TM2 domain of the γ subunit were required to confer high amiloride potency. In summary, we have identified novel pore-lining residues of the γ subunit of ENaC that are important for proper channel gating ...
DiscussionThe interventions of education and medication alerts resulted in a significant increase in the percentage of patients receiving appropriate doses of dilTIAZem and a nonsignificant decrease in the incidence of hypotension. This process-oriented intervention resulted in an improvement in appropriate dilTIAZem doses at our site. Rate control was not statistically significantly different between the 2 groups. Long-term sustainability of this intervention requires further study.
ConclusionsVitamin D insufficiency was prevalent in both newly diagnosed axSpA patients and healthy controls. There was no association between vitamin D and pain and disease activity in the newly diagnosed axSpA patients. Monitoring vitamin D levels is important and early intervention for vitamin D insufficiency is needed, especially in female patients.
ConclusionVacuum assisted dressing is more effective than traditional wound dressing in wound healing of open fractures.
This study aims to reflect on the process of adoption of the preprints publication model, briefly delimiting its history and current use by the national and the international scientific community. Departing from the literature and a consultation of preprint repositories, this reflection intends to highlight the main challenges that preprint implementation will face in the Nursing area, along with its specificities. While considering its benefits for scientific dissemination, this study points out the difficulties that may arise from the implementation of mostly north-American science models in a peripheral country such as ...
RESUMO Objetivo Identificar na literatura a avalia ção da qualidade dos serviços de Atenção Primária à Saúde sob a ótica de cuidadores de crianças por meio da aplicação do PCATool, versão infantil. Método Revisão integrativa realizada nas bases de dados MEDLINE, CINAHL, LILACS, BDENF e Web of Science. Resultados Foram selecionados 17 artigos. Todos os estudos incluídos eram descritivos (100%) e possuíam nível de evidência IV (100%). O componente grau de afiliação (média=7,...
CONCLUSIONS: Neonatal screening with the red reflex is not sufficient to detect intraocular disease. A normal neonatal red reflex does no exclude intraocular disease. It has a specificity of nearly 90%, but its sensitivity is only 56%. Most retinal haemorrhages are undiagnosed as they cannot be detected with a red reflex. PMID: 31826811 [PubMed - as supplied by publisher]
Reaction to claims of study misconduct have dominated the press and social media since the report dropped earlier this week.Medscape Medical News
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