Single-cell classification of foodborne pathogens using hyperspectral microscope imaging coupled with deep learning frameworks

Publication date: 15 April 2020Source: Sensors and Actuators B: Chemical, Volume 309Author(s): Rui Kang, Bosoon Park, Matthew Eady, Qin Ouyang, Kunjie ChenAbstractA high-throughput hyperspectral microscope imaging (HMI) technology with hybrid deep learning (DL) framework defined as “Fusion-Net” was proposed for rapid classification of foodborne bacteria at single-cell level. HMI technology is useful in single-cell characterization, providing spatial, spectral and combined spatial-spectral profiles with high resolution. However, direct analysis of these high-dimensional HMI data is challenging. In this work, HMI data were decomposed into three parts as morphological features, intensity images, and spectral profiles. Multiple advanced DL frameworks including long-short term memory (LSTM) network, deep residual network (ResNet), and one-dimensional convolutional neural network (1D-CNN) were utilized, achieving classification accuracies of 92.2 %, 93.8 %, and 96.2 %, respectively. Taking advantage of fusion strategy, individual DL framework was stacked to form “Fusion-Net” that processed these features simultaneously with improved classification accuracy of up to 98.4 %. Our study demonstrated the ability of DL frameworks to assist HMI technology in single-cell classification as a diagnostic tool for rapid detection of foodborne pathogens.Graphical abstract

Source: Sensors and Actuators B: Chemical - February 11, 2020 Category: Chemistry Source Type: research