The silent hemoglobin variant during capillary electrophoresis: a case report

AbstractHemoglobin (Hb) North Manchester [ β51(D2) Pro→His;HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects HbA1c measurement values such as ion‐exchange high‐performance liquid chromatography (ion‐exchange HPLC) in patients with diabetes. This variant was first detected in UK in 1998. Here, we describe the first case involving Hb North Manchester detected incid entally in a patient with type 2 diabetes in Northern China. The Hb variant was discovered by ion‐exchange HPLC, yet capillary electrophoresis of both HbA1c program and Hb program failed to detect it. Subsequently, Sanger sequencing was conducted to help identify the Hb variant.
Source: Journal of Diabetes Investigation - Category: Endocrinology Authors: Tags: CASE REPORT Source Type: research