An Ultimately Tufnellian Look At Oxford Nanopore R10.0 Homopolymer Performance

Homopolymeric sequences have never been easy for any sequencing platform, but single molecule sequencers struggle the most with this.   Oxford Nanopore has made remarkable strides in both raw an consensus accuracy via chemistry and software improvements, but still is challenged by systematic problems with homopolymers.  The R10 series of pores is intended to significantly improve performance by having a longer narrow region to i nteract with more bases, and at the Nanopore Community Meeting there were several slides touting improved performance.  Nanopore's slides have an X-axis that goes to 8.   By happy circumstance, around that time we generated a large dataset on R10 and got results very similar to ONT's.   Plus there's adatasetavailable from Mads Albertsen's group to support theirupdated pre-print on using Unique Molecular Identifiers (UMIs) to generate high quality consensus sequences.   But our internal dataset is  the best, as ours goes to eleven!Read more »
Source: Omics! Omics! - Category: Bioinformatics Authors: Source Type: blogs