A novel immunochromatographic assay using ultramarine blue particles as visible label for quantitative detection of hepatitis B virus surface antigen.

A novel immunochromatographic assay using ultramarine blue particles as visible label for quantitative detection of hepatitis B virus surface antigen. Anal Chim Acta. 2020 Feb 15;1098:140-147 Authors: Liu J, Yu Q, Zhao G, Dou W Abstract Ultramarine blue particles as a novel visible label has been used to develop immunochromatographic assay (ICA). The ultramarine blue particles, as a sodalite mineral with formula: (Na,Ca)8[(S,Cl,SO4,OH)2(Al6Si6O24)], can generate a blue visible signal were used as a label for ICA. Ultramarine blue particles were applied to a sandwich immunoassay to detect hepatitis B virus surface antigen (HBsAg). Ultramarine blue particles were separated from ultramarine blue industrial product by centrifugation. The polyacrylic acid (PAA) was used to modify the carboxyl group on the surface of ultramarine blue particles. The goat anti-HBsAg monoclonal antibody was modified on ultramarine blue particles by EDC/NHS activation of the carboxyl groups. In the presence of HBsAg, the immune ultramarine blue particles were bound on test line zone and forming a blue line on ICA strip which was directly readout by naked eye and quantitatively measured by Image J software. Under optimal conditions, the color depth of test line was linearly correlated with the concentration of HBsAg in concentration range from 1 to 50 ng mL-1. The calibration equation was y = 385.796 + 97.2298x (R2 = 0.9872), with limit of de...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research

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For more information go tohttps://www.cc.nih.gov/about/news/grcurrent.htmlAir date: 3/4/2020 12:00:00 PM
Source: Videocast - All Events - Category: General Medicine Tags: Upcoming Events Source Type: video
Dr Hou and colleagues1 proposed the management algorithm to interrupt mother-to-infant transmission of hepatitis B virus (HBV) in a recent issue of Clinical Gastroenterology and Hepatology. Although the article provides valuable information in preventing mother-to-infant transmission of HBV, I have some concerns about this article.
Source: Clinical Gastroenterology and Hepatology - Category: Gastroenterology Authors: Tags: Letter to the Editor Source Type: research
Publication date: Available online 17 February 2020Source: Antiviral ResearchAuthor(s): Yingying Dou, Diahann T.S.L. Jansen, Aniek van den Bosch, Robert A. de Man, Nadine van Montfoort, Can Araman, Sander I. van Kasteren, Gijs G. Zom, Willem-Jan Krebber, Cornelis J.M. Melief, Andrea M. Woltman, Sonja I. BuschowAbstractSynthetic long peptide (SLP) vaccination is a promising new treatment strategy for patients with a chronic hepatitis B virus (HBV) infection. We have previously shown that a prototype HBV-core protein derived SLP was capable of boosting CD4+ and CD8+ T cell responses in the presence of a TLR2-ligand in chroni...
Source: Antiviral Therapy - Category: Virology Source Type: research
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Source: Medscape Hiv-Aids Headlines - Category: Infectious Diseases Tags: Gastroenterology Journal Article Source Type: news
Interferon-alpha (IFN α) is a first-line treatment option for chronic hepatitis B virus (HBV) infection, but the severe systemic side-effects limited its clinical application. Interferon-lambda (IFNλ) with comp...
Source: BMC Cell Biology - Category: Cytology Authors: Tags: Research article Source Type: research
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Taxi driver with diabetes and hepatitis B is fifth fatality outside mainland ChinaA taxi driver has died from the coronavirus in Taiwan, marking the first such death on the island and the fifth fatality outside mainland China from an epidemic that has curbed travel and disrupted global supply chains.The health minister, Chen Shih-chung, said during a news conference on Sunday that the deceased was a 61-year-old man who had diabetes and hepatitis B. Taiwan has to date reported 20 confirmed cases.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Coronavirus outbreak Taiwan China Infectious diseases Medical research Science World news Asia Pacific Source Type: news
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Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Bacterial, viral, or overall infection was associated with increased Parkinson's disease risk.Helicobacter pylori, hepatitis C virus, Malassezia, and pneumoniae infection were positively associated with the risk of Parkinson's disease. However, infection of herpes virus, hepatitis B virus, pertussis, scarlet fever, mumps, chicken pox, influenza virus, measles, and German measles was not. AbstractAimsRecent studies showed that patients with various bacterial, viral, and fungal infections might be at increased risk of Parkinson's disease (PD). However, the risk of PD in patients with each specific infection varied. This meta...
Source: Brain and Behavior - Category: Neurology Authors: Tags: ORIGINAL RESEARCH Source Type: research
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Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
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