Subcritical water extraction of phenolic antioxidants with improved α-amylase and α-glucosidase inhibitory activities from exocarps of Castanea mollissima Blume

Publication date: Available online 2 January 2020Source: The Journal of Supercritical FluidsAuthor(s): Xuanchi Liu, Yanhui Wang, Jiachan Zhang, Linlin Yan, Shiqi Liu, Ahmed Aboueloyoun Taha, Jing Wang, Chao MaAbstractsHerein, subcritical water extraction (SWE) technology was applied for the recovery of phenolics from chestnut exocarps. Results showed that extracts obtained by SWE had significantly higher extraction yield (Ye), total phenolic contents (TPC), as well as stronger antioxidant (AAs), α-amylase and α-glucosidase inhibitory activities than conventional techniques. The optimal temperature of SWE for reaching the highest Ye, TPC and AAs was 220 °C, while the strongest α-amylase and α-glucosidase inhibitory activities were observed at 120 °C. UPLC-MS/MS, heatmap and radar chart analysis revealed that the phenolic profiles of different extracts varied markedly with extraction methods, and SWE extract at 120 °C had potential application for glycemic control by non-competitively inhibiting α-amylase and/or α-glucosidase. The major components contributed to the inhibitory activities were putative chlorogenic acid and flavonoids, such as rutin and quercetin, etc. These observations suggest that SWE is a promising technology for obtaining bioactive phenolics from chestnut exocarps.Graphical Abstract
Source: The Journal of Supercritical Fluids - Category: Chemistry Source Type: research