Study of structural and molecular interaction for the catalytic activity of cellulases: An insight in cellulose hydrolysis for higher bioethanol yield

Publication date: 15 March 2020Source: Journal of Molecular Structure, Volume 1204Author(s): Manish Paul, Gayatree Panda, Pradeep Kumar Das Mohapatra, Hrudayanath ThatoiAbstractComparative sequence alignment, molecular modeling and docking analyses were performed taking 5 microbial (3 bacteria and 2 fungi) cellulase enzymes and its substrate β-D glucose in order to understand the enzyme-substrate interaction and binding affinity associated with cellulose hydrolysis. Amino acids such as Val114, Ala255, Val288, Val289, and Ser301 of Streptomyces sp. Endoglucanase-1 shown to bind with β-D glucose. The binding energy between the modeled cellulase and substrate complex resulted that Endoglucanase-1 from Streptomyces sp. has the most binding affinity of −5.61 kcal/mol for the cellulose unit. Deleterious mutations in cellulase enzyme, E133A and H98A reported previously in Dickeya dadantii have been designed and validated using in silico approach. The number of hydrogen bond interactions reported in wild type Endoglucanase-1 from Streptomyces sp. was 22, whereas E133A and H98A mutant cellulase from Dickeya dadantii, the interaction number comes down to 5 and 6 respectively. Binding energy recorded for these two mutants is −4.52 and −4.91 respectively which is less than its wild type form. Phylogenetic tree analysis suggests that the modeled cellulase enzyme from Streptomyces sp. is closely related to the cellulase from Thermobispora bis...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research

Related Links:

ConclusionSubstantial agreement exists among experts regarding many strong recommendations for the improvement of practice concerning the use of muscle relaxants and reversal agents during anaesthesia. In particular, the French Society of Anaesthesia and Intensive Care (SFAR) recommends the use of a device to monitor neuromuscular blockade throughout anaesthesia.
Source: Anaesthesia, Critical Care and Pain Medicine - Category: Anesthesiology Source Type: research
Publication date: January 2020Source: Anaesthesia &Intensive Care Medicine, Volume 21, Issue 1Author(s):
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Publication date: January 2020Source: Anaesthesia &Intensive Care Medicine, Volume 21, Issue 1Author(s):
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Date: Thursday, 02 06, 2020; Speaker: Dr. Jean-Marc Egly , Institut de Genetique et de Biologie Mol éculaire et Cellulaire (IGBMC); Building 37; 4041
Source: NIH Calendar of Events - Category: American Health Source Type: events
Date: Tuesday, 04 07, 2020; Speaker: Brad Walters, Ph.D., Assistant Professor, Dept. of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center; 35A; 610
Source: NIH Calendar of Events - Category: American Health Source Type: events
Date: Tuesday, 04 28, 2020; Speaker: Uli Mueller, Ph.D., Bloomberg Distinguished Professor of Neuroscience and Biology, Johns Hopkins University; 49; 1A51/1A59
Source: NIH Calendar of Events - Category: American Health Source Type: events
Date: Friday, 01 17, 2020; Speaker: Dr. Nihal Altan-Bonnet, Senior Investigator, NHLBI; Dr. Justin Taraska, Senior Investigator, NHLBI; 50; 2nd Floor Lobby
Source: NIH Calendar of Events - Category: American Health Source Type: events
Date: Friday, 01 24, 2020; Speaker: Peter D. Crompton, M.D., M.P.H. , Chief, Malaria Infection Biology and Immunity Unit , Laboratory of Immunogenetics, NIAID ; Building 1; Wilson Hall ; Videocast Event
Source: NIH Calendar of Events - Category: American Health Source Type: events
Date: Friday, 01 24, 2020; Speaker: Diane L. Bolton, Ph.D., Chief, Animal Models&Pathogenesis Section, US Military HIV Research Program; 33; 1N09
Source: NIH Calendar of Events - Category: American Health Source Type: events
.
Source: Human Vaccines and Immunotherapeutics - Category: Allergy & Immunology Authors: Source Type: research
More News: Molecular Biology | Study