Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging.

Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging. Methods Mol Biol. 2020;2071:209-220 Authors: Pavlou G, Tardieux I Abstract Host cell invasion by Toxoplasma gondii/T. gondii tachyzoites is an obligate but complex multistep process occurring in second-scale. To capture the dynamic nature of the whole entry process requires fast and high-resolution live cell imaging. Recent advances in T. gondii/host cell genome editing and in quantitative live cell imaging-image acquisition and processing included-provide a systematic way to accurately phenotype T. gondii tachyzoite invasive behaviour and to highlight any variation or default from a standard scenario. Therefore, applying these combined strategies allows gaining deeper insights into the complex mechanisms underlying host cell invasion. PMID: 31758455 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research