Expression patterns of three JAK–STAT pathway genes in feather follicle development during chicken embryogenesis

Publication date: Available online 20 November 2019Source: Gene Expression PatternsAuthor(s): Yingfeng Tao, Xiaoliu Zhou, Zhiwei Liu, Xiaokang Zhang, Yangfan Nie, Xinting Zheng, Shaomei Li, Xuewen Hu, Ge Yang, Qianqian Zhao, Chunyan MouAbstractThe Janus kinase (JAK)–signal transducer and activator of transcription (STAT) (JAK–STAT) pathway is shown to restrain the hair follicles in catagen and telogen and prevent anagen reentry in murine hair follicle cycling. The early roles of JAK-STAT pathway genes in skin development remain uncharacterized in mouse and chicken models. Here, we revealed the expression patterns of three JAK-STAT pathway genes (JAK1, JAK2, and TYK2) in chicken embryonic skin at E6–E10 stages which are key to feather follicle morphogenesis. Multiple sequence alignment of the three genes from chicken and other species all showed a closely related homology with birds like quail and goose. Whole mount in situ hybridization (WISH) revealed weak expression of JAK1, JAK2, and TYK2 in chicken skin at E6 and E7, and followed with the focally restricted signals in the feather follicles of neck and body skin located dorsally at E8 for JAK1, E9 for TYK2 and E10 for JAK2 gene. All three genes displayed stronger expression in feather follicles of neck skin than that of body skin. The expression levels of JAK1 and TYK2 were much stronger than those of JAK2. Quantitative real-time PCR (qRT-PCR) analysis revealed the increased expression tendency for JAK2 both in the n...

Source: Gene Expression Patterns - November 22, 2019 Category: Genetics & Stem Cells Source Type: research