Selection and validation of castor bean (Ricinus communis) reference genes for quantitative PCR (RT-qPCR) in developing and germinating seeds and expression pattern of four ricin-family genes
This study aims to expand the set of internal control genes used for RT-qPCR experiments with Castor bean (Ricinus communis) seeds by evaluating candidate genes across several seed tissues and developmental stages. Nine reference genes were selected, including actin-11 (ACT11), tubulin alpha-2 (Tα2), elongation factor 1-alpha (EF1-α), protein phosphatase 2A-2 (PP2A2), polyubiquitin-3 (PUB3) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Biological samples consisted of R. communis seeds in 15 stages of maturation and germination. We demonstrate that PP2A2, PUB3 and EF1-α are the most stably expresse...
Source: Gene Expression Patterns - September 18, 2019 Category: Genetics & Stem Cells Source Type: research

Ontogeny and tissue-specific expression of immune-relevant genes in Catla catla (Hamilton)
Publication date: Available online 14 September 2019Source: Gene Expression PatternsAuthor(s): P. Arya, P.K. Pradhan, Anutosh Paria, R. Sharma, Dev K. Verma, Ravindra, G. Rathore, N. SoodAbstractIndia is the second largest fish producing country in the world with production of 12.6 million tonnes (mt) in 2017–18, and Indian major carps (IMCs) share bulk of this fish production. Catla, Catla catla is the fastest growing species among IMCs. However, the survival rate of catla during larval rearing is normally lower than the other IMCs i.e rohu Labeo rohita, and mrigal Cirrhinus mrigala. Continuous efforts are devoted f...
Source: Gene Expression Patterns - September 14, 2019 Category: Genetics & Stem Cells Source Type: research

Comparative gene expression analysis of the engulfment and cell motility (ELMO) protein family in the mouse brain
Publication date: Available online 12 September 2019Source: Gene Expression PatternsAuthor(s): Yumi Sato, Akira Sato, Shota Mizuno, Jun-na Hirota, Shuhei Fujima, Chiaki Ishii, Yoshitake Sano, Teiichi FuruichiAbstractEngulfment and cell motility (ELMO) proteins bind to Dock180, a guanine nucleotide exchange factor (GEF) of the Rac family, and regulate GEF activity. The resultant ELMO/Dock180/Rac module regulates cytoskeletal reorganization responsible for the engulfment of apoptotic cells, cell migration, and neurite extension. The expression and function of Elmo family proteins in the nervous system, however, are not yet f...
Source: Gene Expression Patterns - September 13, 2019 Category: Genetics & Stem Cells Source Type: research

Spatiotemporal expression profile of embryonic and adult ankyrin repeat and EF-hand domain containing protein 1-encoding genes ankef1a and ankef1b in zebrafish
Publication date: Available online 12 September 2019Source: Gene Expression PatternsAuthor(s): Jeffrey G. Daniel, Jennifer R. PanizziAbstractRecent human next-generation sequencing (NGS) studies indicate a correlation between ANKEF1 (ankyrin repeat and EF-hand domain containing protein 1) expression and cilia formation or function. Additionally, a single study conducted in the African clawed frog (Xenopus laevis) showed ankef1 is down-regulated after pharmacological fibroblast growth factor (FGF) inhibition and plays a role in protocadherin-mediated cell protrusion and adhesion. That study also revealed a critical role for...
Source: Gene Expression Patterns - September 12, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: September 2019Source: Gene Expression Patterns, Volume 33Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - August 25, 2019 Category: Genetics & Stem Cells Source Type: research

Expression of transcription factors and signaling molecules in the cerebellar granule cell development
This study provides a basis for future studies on the cerebellar GC development and medulloblastoma. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - August 20, 2019 Category: Genetics & Stem Cells Source Type: research

Expression of Lhx6 in the adult and developing mouse retina
ConclusionLhx6 was expressed in RGCs and ACs in both the adult and developing mouse retina. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - July 31, 2019 Category: Genetics & Stem Cells Source Type: research

Early expression of Tubulin Beta-III in avian cranial neural crest cells
Publication date: Available online 29 July 2019Source: Gene Expression PatternsAuthor(s): Jose Chacon, Crystal D. RogersAbstractNeural crest cells are a transient stem-like cell population that forms in the dorsal neural tube of vertebrate embryos and then migrates to various locations to differentiate into diverse derivatives such as craniofacial bone, cartilage, and the enteric and peripheral nervous systems. The current dogma of neural crest cell development suggests that there is a specific hierarchical gene regulatory network (GRN) that controls the induction, specification, and differentiation of these cells at speci...
Source: Gene Expression Patterns - July 31, 2019 Category: Genetics & Stem Cells Source Type: research

Spatial analysis of RECK, MT1-MMP, and TIMP-2 proteins during early Xenopus laevis development
Publication date: Available online 26 July 2019Source: Gene Expression PatternsAuthor(s): Jessica A. Willson, Sashko DamjanovskiAbstractProper cell-cell and cell-ECM interactions are vital for cell migration and patterning of the vertebrate embryo. MMPs and their inhibitors, RECK and TIMPs, are all differentially expressed during embryogenesis to regulate such ECM remodeling and cell interactions. MT1-MMP, RECK, and TIMP-2 are unique amongst other ECM-regulating proteins as they act in the pericellular space. Past studies have shown that RECK and TIMP-2 interact with MT1-MMP on the cell surface, thereby influencing cell be...
Source: Gene Expression Patterns - July 27, 2019 Category: Genetics & Stem Cells Source Type: research

Hectd1 is essential for embryogenesis in mice
Publication date: Available online 10 July 2019Source: Gene Expression PatternsAuthor(s): Donato D'Alonzo, Fabienne Hélène Emch, Xiaoli Shen, Elisabeth Bruder, Christian De Geyter, Hong ZhangAbstractMany aspects of the functional role of the E3 ubiquitin ligase Hectd1 in embryogenesis and in cell biology still remain to be elucidated. In order to contribute to this task we now report the generation of a new transgenic mouse model for Hectd1 using the gene trap strategy. The HECT domain deletion mutant mouse was created by inserting a β-geo cassette into the Hectd1 locus. Mice homozygous for Hectd1-mutant...
Source: Gene Expression Patterns - July 10, 2019 Category: Genetics & Stem Cells Source Type: research

Genome imprinting in stem cells: A mini-review
Publication date: Available online 4 July 2019Source: Gene Expression PatternsAuthor(s): Rasoul Godini, Keyvan Karami, Hossein FallahiAbstractGenomic imprinting is an epigenetic process result in silencing of one of the two alleles (maternal or paternal) based on the parent of origin. Dysregulation of imprinted genes results in detectable developmental and differential abnormalities. Epigenetics erasure is required for resetting the cell identity to a ground state during the production of induced pluripotent stem (iPS) cells from somatic cells. There are some contradictory reports regarding the status of the imprinting mar...
Source: Gene Expression Patterns - July 6, 2019 Category: Genetics & Stem Cells Source Type: research

Expression pattern of Kmt2d in murine craniofacial tissues
Publication date: Available online 19 June 2019Source: Gene Expression PatternsAuthor(s): Chunmin Dong, Meenakshi Umar, Garrett Bartoletti, Apurva Gahankari, Lauren Fidelak, Fenglei HeAbstractFormation of the calvaria is a multi-staged process and is regulated by multiple genetic factors. Disruption of normal calvarial development usually causes craniosynostosis, a prevalent birth defect characterized by premature fusion of calvarial bone. Recent studies have identified mutations of KMT2D allele in patients with craniosynostosis, indicating a potential role for Kmt2d in calvarial development. KMT2D mutations have also been...
Source: Gene Expression Patterns - June 21, 2019 Category: Genetics & Stem Cells Source Type: research

Primary cilia in murine palatal rugae development
Publication date: Available online 19 June 2019Source: Gene Expression PatternsAuthor(s): Mayuko Nakaniwa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Fumiya Meguro, Maeda Takeyasu, Atsushi OhazamaAbstractPeriodic patterning of iterative structures is a fundamental process during embryonic development, since these structures are diverse across the animal kingdom. Therefore, elucidating the molecular mechanisms in the formation of these structures promotes understanding of the process of organogenesis. Periodically patterned ridges, palatal rugae (situated on the hard palate of mammals), are an excellent experimental...
Source: Gene Expression Patterns - June 19, 2019 Category: Genetics & Stem Cells Source Type: research

Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode Caenorhabditis elegans
Publication date: Available online 15 June 2019Source: Gene Expression PatternsAuthor(s): Anton Karabinos, Ekkehard Schulze, Ralf BaumeisterAbstractThe multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric...
Source: Gene Expression Patterns - June 15, 2019 Category: Genetics & Stem Cells Source Type: research

Expression pattern of ABCDE model genes in floral organs of bolting garlic clone
Publication date: Available online 13 June 2019Source: Gene Expression PatternsAuthor(s): Fahimeh Ghaemizadeh, Farshad Dashti, Alireza ShafeiniaAbstractA correct understanding of the ABCDE model genes expression, especially genes involved in the development of the reproductive floral organs, in bolting and fertile garlic clones improves the process of its fertilization and breeding programs. Therefore, Real-Time PCR was employed to evaluate the temporal and spatial expression patterns of some floral organ identity genes in the inflorescence and different floret organs in the two stages of green and purple florets of boltin...
Source: Gene Expression Patterns - June 13, 2019 Category: Genetics & Stem Cells Source Type: research

Evaluation and validation of experimental condition-specific reference genes for normalization of gene expression in Asia II-I Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)
Publication date: Available online 8 June 2019Source: Gene Expression PatternsAuthor(s): Ramandeep Kaur, Mridula Gupta, Satnam Singh, Suneet PandherAbstractFunctional genomics in whitefly, Bemisia tabaci is gaining impetus due to its polyphagous nature, worldwide distribution and recently sequenced whole genome. These studies require an in-depth evaluation and validation of reference genes in different development stages and variable experimental setups. Normalization with reference genes is an essential step in the gene expression studies. Rather than selecting a reference gene empirically, the suitability of these genes ...
Source: Gene Expression Patterns - June 9, 2019 Category: Genetics & Stem Cells Source Type: research

Expression of the guanine nucleotide exchange factor, RAPGEF5, during mouse and human embryogenesis
Publication date: Available online 1 June 2019Source: Gene Expression PatternsAuthor(s): Reham Alharatani, John N. Griffin, Karen J. LiuAbstractRap GTPases mediate fundamental cellular processes, including cell adhesion, migration and intracellular signal transduction. The subcellular activity of these GTPases is regulated by dedicated activators (guanine nucleotide exchange factors, GEFs) and deactivators (GTPase-activating proteins, GAPs). RAPGEF5 is a potent activator of Rap proteins and mutations in RAPGEF5 have been linked to both neurological disorders and congenital heart disease. In the frog model, Xenopus tropical...
Source: Gene Expression Patterns - June 1, 2019 Category: Genetics & Stem Cells Source Type: research

Developmental expression patterns of fosl genes in Xenopus tropicalis
Publication date: Available online 21 May 2019Source: Gene Expression PatternsAuthor(s): Xiao-Fang Guo, Zhou Zhang, Li Zheng, Yi-Min Zhou, Hai-Yan Wu, Chi-Qian Liang, Hui Zhao, Dong-Qing Cai, Xu-Feng QiAbstractFos-like antigens (Fosl) including Fosl1 and Fosl2 exclusively heterodimerize with Jun members to form AP-1 complex, thereby participating in various cellular progresses including cell cycle regulation. However, expression patterns of these two genes during embryonic development remains largely unknown. In the present study, both temporal and spatial expression patterns of fosl1 and fosl2 were examined during embryon...
Source: Gene Expression Patterns - May 22, 2019 Category: Genetics & Stem Cells Source Type: research

Altered levels of hsromega lncRNAs further enhance ras signaling during ectopically activated ras induced R7 differentiation in Drosophila
Publication date: Available online 13 May 2019Source: Gene Expression PatternsAuthor(s): Mukulika Ray, Gunjan Singh, Subhash C. LakhotiaAbstractWe exploited the high Ras activity induced differentiation of supernumerary R7 cells in Drosophila eyes to examine if hsrω lncRNAs influence active Ras signaling. Surprisingly, either down- or up-regulation of hsrω lncRNAs in sev-GAL4>RasV12 expressing eye discs resulted in complete pupal lethality and substantially greater increase in R7 photoreceptor number at the expense of cone cells. Enhanced nuclear p-MAPK and presence of sev-GAL4 driven RasV12 bound RafRBDFLAG...
Source: Gene Expression Patterns - May 13, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: June 2019Source: Gene Expression Patterns, Volume 32Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - May 13, 2019 Category: Genetics & Stem Cells Source Type: research

Spatio-temporal expression profile of sirtuins during aging of the annual fish Nothobranchius furzeri
Publication date: Available online 7 May 2019Source: Gene Expression PatternsAuthor(s): Julijan Kabiljo, Christina Murko, Oliver Pusch, Gordin ZupkovitzAbstractThe founding member of the sirtuin family, yeast Sir2, was the first evolutionarily conserved gene to be identified as a regulator of longevity. Sirtuins constitute a protein family of metabolic sensors, translating changes in NAD + levels into adaptive responses, thereby acting as crucial regulators of the network that controls energy homeostasis and as such determines healthspan. In mammals the sirtuin family comprises seven proteins, SIRT1-SIRT7, which ...
Source: Gene Expression Patterns - May 8, 2019 Category: Genetics & Stem Cells Source Type: research

Evolution and expression of the zebrafish unc119 paralogues indicates a conserved role in cilia
Publication date: Available online 2 May 2019Source: Gene Expression PatternsAuthor(s): Francesca Jean, David PilgrimAbstractUNC119 proteins are required for ciliary trafficking in a process called lipidated protein intraflagellar targeting (LIFT) or through vesicle transport. However, although unc119 has been studied in a variety of contexts, either organismal constraints or genetic redundancy has largely restricted their study in ciliary contexts. One possible solution for this is to use the zebrafish, however, the unc119 genes have not been well described in this species. In our study, we show in a condensed species tre...
Source: Gene Expression Patterns - May 4, 2019 Category: Genetics & Stem Cells Source Type: research

Bmp signaling in molar cusp formation
Publication date: Available online 11 April 2019Source: Gene Expression PatternsAuthor(s): Fumiya Meguro, Thantrira Porntaveetus, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Yoshito Kakihara, Makio Saeki, Koichi Tabeta, John A. Kessler, Takeyasu Maeda, Atsushi OhazamaAbstractTooth cusp is a crucial structure, since the shape of the molar tooth is determined by number, shape, and size of the cusp. Bone morphogenetic protein (Bmp) signaling is known to play a critical role in tooth development, including in initiation. However, it remains unclear whether Bmp signaling is also involved in cusp formation. To address thi...
Source: Gene Expression Patterns - April 11, 2019 Category: Genetics & Stem Cells Source Type: research

Expression analysis of the aquaporins during zebrafish embryonic development
Publication date: Available online 2 April 2019Source: Gene Expression PatternsAuthor(s): Yinyin Qin, Shiping Wang, Xuchu Duan, Dong LiuAbstractThe aquaporins are integral membrane proteins from a larger family of major intrinsic protein (MIP) that form pores in the membrane of cells. These proteins selectively transport water and other small uncharged solutes across cell plasma membranes. The organization of water within cells and tissues is fundamental to life, and the aquaporins play an important role in serving as the plumbing system for cells. As many as thirteen mammalian AQPs have been characterized, which have been...
Source: Gene Expression Patterns - April 4, 2019 Category: Genetics & Stem Cells Source Type: research

Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm
Publication date: Available online 30 March 2019Source: Gene Expression PatternsAuthor(s): Emily A. Baumgartner, Zachary J. Compton, Spencer Evans, J. Topczewski, Elizabeth E. LeClairAbstractWe have cloned and characterized an intronic fragment of zebrafish lymphocyte cytosolic protein 1 (lcp1, also called L-plastin) that drives expression to the zebrafish enveloping layer (EVL). L-plastin is a calcium-dependent actin-bundling protein belonging to the plastin/fimbrin family of proteins, and is necessary for the proper migration and attachment of several adult cell types, including leukocytes and osteoclasts. Unexpectedly, ...
Source: Gene Expression Patterns - March 31, 2019 Category: Genetics & Stem Cells Source Type: research

Spatiotemporal expression pattern of Sjfz7 and its expression comparison with other frizzled family genes in developmental stages of Schistosoma japonicum
Publication date: Available online 6 March 2019Source: Gene Expression PatternsAuthor(s): Zhongxue Ye, Jingxiu Xu, Xingang Feng, Yingying Jia, Zhiqiang Fu, Yang Hong, Hao Li, Ke Lu, Jiaojiao Lin, Mingxin Song, Liqun Wang, Chunxiu YuanAbstractWnts are secreted signaling molecules that are implicated in a variety of growth-related processes. Frizzled proteins have been identified as receptors for Wnt ligands in vertebrates and invertebrates, but a functional role for dioecious flatworm Frizzleds has not been determined. To evaluate the endogenous role of Frizzled proteins during development, we have identified and characteri...
Source: Gene Expression Patterns - March 8, 2019 Category: Genetics & Stem Cells Source Type: research

GTP binding protein 10 is a member of the OBG family of proteins and is differentially expressed in the early Xenopus embryo
Publication date: Available online 1 March 2019Source: Gene Expression PatternsAuthor(s): Razhan Jerry, Jessica Sullivan-Brown, Michael D. Yoder (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - March 4, 2019 Category: Genetics & Stem Cells Source Type: research

Evolution of the Rho guanine nucleotide exchange factors Kalirin and Trio and their gene expression in Xenopus development
Publication date: Available online 4 March 2019Source: Gene Expression PatternsAuthor(s): Marie-Claire Kratzer, Laura England, David Apel, Monika Hassel, Annette BorchersAbstractGuanine nucleotide exchange factors (GEFs) activate Rho GTPases by accelerating their GDP/GTP exchange. Trio and its paralog Kalirin (Kalrn) are unique members of the Rho-GEFs that harbor three catalytic domains: two functional GEF domains and a serine/threonine kinase domain. The N-terminal GEF domain activates Rac1 and RhoG GTPases, while the C-terminal GEF domain acts specifically on RhoA. Trio and Kalrn have an evolutionary conserved function i...
Source: Gene Expression Patterns - March 4, 2019 Category: Genetics & Stem Cells Source Type: research

An in situ hybridization study of MMP-2, -9, -13, -14, TIMP-1, and -2 mRNA in fetal mouse mandibular condylar cartilage as compared with limb bud cartilage
Publication date: Available online 27 February 2019Source: Gene Expression PatternsAuthor(s): Masato Takahashi, Kaoru Fujikawa, Randilini Angammana, Shunichi ShibataAbstractThe main purpose of this in situ hybridization study was to investigate MMPs and TIMPs mRNA expression in developing mandibular condylar cartilage and limb bud cartilage. At E14.0, MMP-2, -14, TIMP-1 and -2 mRNAs were expressed in the periosteum of mandibular bone, and in the condylar anlage. At E15.0 MMP-2, -14, TIMP-1 and -2 mRNAs were expressed in the perichondrium of newly formed condylar cartilage and the periosteum of developing bone collar, where...
Source: Gene Expression Patterns - February 27, 2019 Category: Genetics & Stem Cells Source Type: research

Expression of the heparin-binding growth factors Midkine and pleiotrophin during ocular development
In this study, we demonstrate by section in situ hybridization, the spatiotemporal expression of MDK and PTN during ocular development in chick and mouse. We show that MDK and PTN are expressed in dynamic patterns that overlap in a few non-neuronal tissues in the anterior eye and in neuronal cell layers of the posterior eye. We show that the expression patterns of MDK and PTN are only conserved in a few tissues in chick and mouse but they overlap with the expression of some of their receptors LRP1, RPTPZ, ALK, NOTCH2, ITGβ1, SDC1, and SDC3. The dynamic expression patterns of MDK, PTN and their receptors suggest that t...
Source: Gene Expression Patterns - February 27, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: January 2019Source: Gene Expression Patterns, Volume 31Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - February 13, 2019 Category: Genetics & Stem Cells Source Type: research

Regionalized nervous system in Hydra and the mechanism of its development
In this study we show how it is formed and maintained, focusing on the neuropeptide Hym-176 gene and its paralogs. First, we demonstrate that four axially localized neuron subsets that express different combination of the neuropeptide Hym-176 gene and its paralogs cover almost an entire body, forming a regionalized nervous system in Hydra. Second, we show that positional information governed by the Wnt signaling pathway plays a key role in determining the regional specificity of the neuron subsets as is the case in bilaterians. Finally, we demonstrated two basic mechanisms, regionally restricted new differentiation and phe...
Source: Gene Expression Patterns - January 23, 2019 Category: Genetics & Stem Cells Source Type: research

Expression of RNA-binding protein Rbfox1l demarcates a restricted population of dorsal telencephalic neurons within the adult zebrafish brain
In this study, we characterize Rbfox1l and Rbfox2 expression in the adult zebrafish brain. While Rbfox2 is expressed broadly, Rbfox1l is expressed in restricted populations of neurons in the dorsal telencephalon and cerebellum. In the dorsal telencephalon, Rbfox1l is expressed in a specific population of neurons spanning Dm and Dc regions. In the cerebellum, Rbfox1l and Rbfox2 are expressed in the Purkinje cell layer, reminiscent of Rbfox1 and Rbfox2 expression in the mammalian cerebellum. Our findings motivate future studies of Rbfox function in the zebrafish brain. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - January 9, 2019 Category: Genetics & Stem Cells Source Type: research

Expression of teneurin-m/odd Oz during segmentation in the beetle Tribolium castaneum
Publication date: Available online 7 January 2019Source: Gene Expression PatternsAuthor(s): Sil Jin, Jiyun O, Frank Stellabotte, Susan J. Brown, Chong Pyo ChoeAbstractThe pair-rule gene teneurin-m/odd Oz (ten-m/odz) is required for the patterning of alternate segment boundaries in the early Drosophila embryo. Mutant phenotypes of ten-m/odz display a typical pair-rule phenotype in which odd-numbered segments are eliminated. Consistent with its pair-rule function, Ten-m/Odz protein is expressed in a seven-stripe pattern before the onset of gastrulation. While expression of ten-m/odz orthologs have been characterized in sever...
Source: Gene Expression Patterns - January 8, 2019 Category: Genetics & Stem Cells Source Type: research

Expressional and functional analyses of epididymal SPINKs in mice
Publication date: Available online 25 December 2018Source: Gene Expression PatternsAuthor(s): Juri Jeong, Boyeon Lee, Jihye Kim, Jaehwan Kim, Seong Hyeon Hong, Donghyun Kim, Seungho Choi, Byung-Nam Cho, Chunghee ChoAbstractEpididymal maturation is critical for acquisition of motility and fertilizing capacity by sperm. During epididymal transit, the surface of sperm undergoes prominent sequential changes through interactions with secreted proteins, including protease inhibitors. In the present study, we characterized three epididymis-specific SPINKs (serine protease inhibitors, Kazal-type): SPINK8, SPINK11, and SPINK12. We ...
Source: Gene Expression Patterns - December 25, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: December 2018Source: Gene Expression Patterns, Volume 30Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - November 29, 2018 Category: Genetics & Stem Cells Source Type: research

Developmental expression patterns of protein kinase A catalytic subunits in zebrafish
Publication date: Available online 20 November 2018Source: Gene Expression PatternsAuthor(s): Sarah E. Emerson, Benjamin K. Grebber, Morgan McNelllis, Ambrose R. Orr, Paula B. Deming, Alicia M. EbertAbstractProtein kinase A (PKA), also known as cAMP dependent protein kinase, is an essential component of many signaling pathways, many of which regulate key developmental processes. Inactive PKA is a tetrameric holoenzyme, comprised of two catalytic (PRKAC), and two regulatory subunits. Upon cAMP binding, the catalytic subunits are released and thereby activated. There are multiple isoforms of PKA catalytic subunits, but their...
Source: Gene Expression Patterns - November 21, 2018 Category: Genetics & Stem Cells Source Type: research

A tissue-specific enhancer of the C. elegans nhr-67/tailless gene drives coordinated expression in uterine stem cells and the differentiated anchor cell
Publication date: Available online 4 November 2018Source: Gene Expression PatternsAuthor(s): Shari Bodofsky, Katarina Liberatore, Lauren Pioppo, Dominic Lapadula, Lily Thompson, Susanna Birnbaum, George McClung, Akshara Kartik, Sheila Clever, Bruce WightmanAbstractThe nhr-67 nuclear receptor gene of Caenorhabditis elegans encodes the ortholog of the Drosophila tailless and vertebrate Tlx genes. In C. elegans, nhr-67 plays multiple roles in the development of the uterus during L2 and L3 larval stages. Four pre-VU cells are born in the L2 stage and form the precursor complement for the ventral surface of the mature uterus. O...
Source: Gene Expression Patterns - November 6, 2018 Category: Genetics & Stem Cells Source Type: research

Analysis of Brassica napus dehydrins and their Co-Expression regulatory networks in relation to cold stress
Publication date: Available online 6 November 2018Source: Gene Expression PatternsAuthor(s): Khazar Edrisi Maryan, Habibollah Samizadeh Lahiji, Naser Farrokhi, Hassan Hasani KomelehAbstractDehydrins (DHNs) are plant specific cold and drought stress-responsive proteins that belong to late embryogenesis abundant (LEA) protein families. B. napus DHNs (BnDHNs) were computationally analyzed to establish gene regulatory- and protein-protein interaction networks. Promoter analyses suggested functionality of phytohormones in BnDHNs gene network. The relative expressions of some BnDHNs were analyzed using qRT-PCR in seedling leaves...
Source: Gene Expression Patterns - November 6, 2018 Category: Genetics & Stem Cells Source Type: research

Expression of trpv channels during Xenopus laevis embryogenesis
Publication date: Available online 13 October 2018Source: Gene Expression PatternsAuthor(s): Chen Dong, Sudip Paudel, Nana Y. Amoh, Margaret S. SahaAbstractTransient receptor potential (TRP) cation channel genes code for an extensive family of conserved proteins responsible for a variety of physiological processes, including sensory perception, ion homeostasis, and chemical signal transduction. The TRP superfamily consists of seven subgroups, one of which is the transient receptor potential vanilloid (trpv) channel family. While trpv channels are relatively well studied in adult vertebrate organisms given their role in fun...
Source: Gene Expression Patterns - October 15, 2018 Category: Genetics & Stem Cells Source Type: research

Class I histone deacetylases in retinal progenitors and differentiating ganglion cells
ConclusionThese results indicate there may be a role for one or more of the Class I HDACs in retinal cell type-specific differentiation. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - September 2, 2018 Category: Genetics & Stem Cells Source Type: research

ALS-associated genes display CNS expression in the developing zebrafish
Publication date: Available online 27 August 2018Source: Gene Expression PatternsAuthor(s): Lauren A. Laboissonniere, Courtney L. Smith, Jacquelyn Mesenbrink, Rebecca Chowdhury, Abbie Burney, Margaret Lang, Matthew Sierra, Amy Stark, Gabriel Maldonado-Casalduc, Madison Muller, Jeffrey M. TrimarchiAbstractAmyotrophic lateral sclerosis (ALS) is characterized by progressive muscle atrophy resulting from the deterioration of motor neurons in the central nervous system (CNS). Recent genome-wide association studies have revealed several genes linked to ALS, further demonstrating the complexity of the disease. The zebrafish (Dani...
Source: Gene Expression Patterns - August 28, 2018 Category: Genetics & Stem Cells Source Type: research

Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis
Publication date: Available online 24 August 2018Source: Gene Expression PatternsAuthor(s): Atsuo Kawahara, Hitoshi Morita, Kanoko Yanagi, Tomoe Ishizaka, Kiyohito Taimatsu, Rie OhgaAbstractMammalian SLURP1 and SLURP2 belong to the Ly-6/uPAR superfamily and are involved in maintaining the physiological integrity of keratinocytes. However, the developmental expression and functions of other Ly-6/uPAR family genes in vertebrates are still obscure. We have isolated novel Ly-6/uPAR family genes slurp-like1 (ly2.3/ly97.3) and slurp-like2 (ly2.2/ly97.2) in zebrafish. Both the Slurp-like1 and Slurp-like2 proteins contain the typi...
Source: Gene Expression Patterns - August 26, 2018 Category: Genetics & Stem Cells Source Type: research

Analysis of the FGFR spatiotemporal expression pattern within the chicken scleral ossicle system
Publication date: Available online 22 August 2018Source: Gene Expression PatternsAuthor(s): Shruti Kumar, Tamara A. Franz-OdendaalAbstractFibroblast Growth Factors (FGFs) play several important roles during organ morphogenesis and act as multi-functional growth factors that bind to their membrane bound receptors (FGFRs) and activate further downstream signalling pathways. Several studies have investigated the function and expression of FGF/FGFRs in endochondral bone development, however, we know little about their role in the development of neural crest derived intramembranous bones. Here, we investigate the expression of ...
Source: Gene Expression Patterns - August 24, 2018 Category: Genetics & Stem Cells Source Type: research

Expression pattern of delta-like 1 homolog in developing sympathetic neurons and chromaffin cells
In this study we have examined the precise spatiotemporal expression of DLK1 in developing sympathoadrenal cells. We show that DLK1 mRNA is highly expressed in early sympathetic neuron progenitors and that its expression depends on the presence of Phox2B. DLK1 expression becomes quickly restricted to a small subpopulation of cells in sympathetic ganglia, while virtually all chromaffin cells in the adrenal medulla and the Organ of Zuckerkandl still express high levels of DLK1 at late gestational stages. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - August 24, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - August 19, 2018 Category: Genetics & Stem Cells Source Type: research

Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis
Publication date: Available online 17 August 2018Source: Gene Expression PatternsAuthor(s): Atsuo Kawahara, Hitoshi Morita, Kanoko Yanagi, Hiroaki Suzuki, Takaaki Mori, Rie Ohga, Kiyohito TaimatsuAbstractThe cocaine- and amphetamine-regulated transcript (CART) genes are involved in the neural regulation of energy homeostasis; however, their developmental expressions and functions are not fully understood in vertebrates. We have identified a novel zebrafish cart-like gene that encodes a protein of 105 amino acids possessing sequence similarity to zebrafish and mammalian CART proteins. RT-PCR analysis revealed that the cart-...
Source: Gene Expression Patterns - August 18, 2018 Category: Genetics & Stem Cells Source Type: research

Molecular characterization of wdr68 gene in embryonic development of Xenopus laevis
Publication date: Available online 17 August 2018Source: Gene Expression PatternsAuthor(s): M. Bonano, E. Martín, M.M. Moreno Ruiz Holgado, G.M. Silenzi Usandivaras, G. Ruiz De Bigliardo, M.J. AybarAbstractWDR68, also known as DCAF7, is a WD40 repeated domain protein highly conserved in eukaryotic organisms in both plants and animals. This protein participates in numerous cellular processes and exerts its function through interaction with other proteins. In the present work, we isolated, sequenced and characterized cDNA corresponding to the wdr68 gene in embryos of the amphibian Xenopus laevis. Syntenic analysis rev...
Source: Gene Expression Patterns - August 18, 2018 Category: Genetics & Stem Cells Source Type: research

Developmental expression of the zebrafish Arf-like small GTPase paralogs arl13a and arl13b
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Ping Song, Brian D. PerkinsAbstractMembers of the Arf-like (Arl) family of small GTP-binding proteins regulate a number of cellular functions and play important roles in cilia structure and signaling. The small GTPase Arl13a is a close paralog to Arl13b, a small GTPase required for normal cilia formation that causes Joubert Syndrome when mutated. As mutation of arl13b causes a slow retinal degeneration in zebrafish (Song et al., 2016), we hypothesized that expression of arl13a may provide functional redundancy. We determined the expressio...
Source: Gene Expression Patterns - July 18, 2018 Category: Genetics & Stem Cells Source Type: research

Astacin gene family of metalloproteinases in planarians: Structural organization and tissue distribution
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Maria Emilia Isolani, Renata Batistoni, Chiara Ippolito, Anna Maria Bianucci, Silvia Marracci, Leonardo RossiAbstractPlanarian flatworms possess extraordinary regenerative capability and body plasticity, which rely on a composite population of stem cells, the neoblasts. Despite impressive advances have been recently achieved in the knowledge of neoblast biology, few is still known about factors that are released by differentiated tissues and influence the neoblast fate. Extracellular matrix (ECM) is a fundamental component of the stem cell nic...
Source: Gene Expression Patterns - July 11, 2018 Category: Genetics & Stem Cells Source Type: research