Hybridization of Oligonucleotides with Hydrophobic Peptide Nucleic Acids Assists Their Cellular Uptake via Aggregate Formation.

Hybridization of Oligonucleotides with Hydrophobic Peptide Nucleic Acids Assists Their Cellular Uptake via Aggregate Formation.
Chembiochem. 2019 Nov 08;:
Authors: Misu S, Kurihara R, Nishihara T, Tanabe K, Kainuma R, Sato R
Abstract
We applied hybridization of hydrophobic peptide nucleic acids (PNA) with oligodeoxynucleotides (ODNs) to their cellular uptake without transfection reagents. We employed a pyrene unit as a hydrophobic functional group and introduced it to the terminal of the PNA strand. The pyrene-tethered PNA (Py PNA) strongly bound with its complementary ODNs to generate amphiphiles; the resulting hybrid formed aggregates that showed efficient cellular uptake and high biological stability. The aggregates, which consisted of a functional DNA aptamer that bound to the Py PNA, penetrated the cell membrane smoothly and exerted its original function in living cells. Thus, Py PNA efficiently assisted the cellular uptake of ODNs without any additives. Here we report the application of PNA to transport ODNs into cells.
PMID: 31702103 [PubMed - as supplied by publisher]

Source: Chembiochem - November 7, 2019 Category: Biochemistry Authors: Misu S, Kurihara R, Nishihara T, Tanabe K, Kainuma R, Sato R Tags: Chembiochem Source Type: research