E-Cigarette Use Increases Susceptibility to Bacterial Infection by Impairment of Human Neutrophil Chemotaxis, Phagocytosis and NET Formation.

E-Cigarette Use Increases Susceptibility to Bacterial Infection by Impairment of Human Neutrophil Chemotaxis, Phagocytosis and NET Formation.
Am J Physiol Cell Physiol. 2019 Oct 30;:
Authors: Corriden R, Moshensky A, Bojanowski CM, Meier A, Chien J, Nelson RK, Crotty Alexander LE
Abstract
E-cigarettes are portrayed as safer relative to conventional tobacco. However, burgeoning evidence suggests that E-cigarettes may adversely affect host defenses. However, the precise mechanisms by which E-cigarette vapor alters innate immune cell function have not been fully elucidated. We determined the effects of E-cigarette exposure on the function and responses to infectious challenge of the most abundant innate immune cell, the neutrophil, using isolated human neutrophils and a mouse model of Gram-negative infection. Our results revealed that human neutrophils exposed to E-cigarette vapor had 4.2-fold reductions in chemotaxis towards the bacterial cell-well component f-Met-Leu-Phe (p < 0.001). F-actin polarization and membrane fluidity were also adversely affected by E-cigarette vapor exposure. E-cigarette exposed human neutrophils exhibited a 48% reduction in production of reactive oxygen species (ROS; p < 0.001). Given the central role of ROS in neutrophil extracellular trap (NET) production, NET production was quantified and E-cigarette vapor exposure was found to reduce NETosis by 3.5-fold (p < 0.01); formulations with and without ...

Source: Am J Physiol Cell Ph... - October 29, 2019 Category: Cytology Authors: Corriden R, Moshensky A, Bojanowski CM, Meier A, Chien J, Nelson RK, Crotty Alexander LE Tags: Am J Physiol Cell Physiol Source Type: research