Development and validation of LC-MS/MS method for urinary 39 steroid (estrogens, androgens, corticoids, and progestins) profiling.

Development and validation of LC-MS/MS method for urinary 39 steroid (estrogens, androgens, corticoids, and progestins) profiling. Biomed Chromatogr. 2019 Oct 27;:e4723 Authors: Son HH, Yun WS, Cho SH Abstract Abnormal production or metabolism of steroid hormones are responsible for the development of endocrine diseases. The accurate quantification of steroid hormones is needed for both research into clinical conditions as well as for diagnostic and monitoring purposes. The improved analytical method for 39 steroid profiling in urine was developed using LC-MS/MS. As a pre-treatment procedure prior to LC-MS/MS analysis, hydrolysis using β-glucuronidase and solid-phase extraction for purifying the samples were performed. Steroids were separated using Waters ACQUITY @ BEH C18 column (2.1 x 100 mm, 1.7 μm) and a mobile phase consisting of eluent A (0.01% formic acid and 1 mM ammonium formate in water) and eluent B (0.01% formic acid and 1 mM ammonium formate in methanol) with a gradient program at a flow rate of 0.4 mL/min. Under the optimized method, the linearity of calibration curves was higher than 0.992. The limits of detection (LOD) at signal to noise (S/N) ratio of 3 were 0.03-90 ng/mL. The developed novel LC-MS/MS method can quantitatively profile 39 steroids in a single analytical run. Steroid profiling based on quantitative results could improve the diagnosis and monitoring of hormone-dependent diseases. PMID: 3165604...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research