Quantification of fenoprofen in human plasma using UHPLC-tandem mass spectrometry for pharmacokinetic study in healthy subjects.

Quantification of fenoprofen in human plasma using UHPLC-tandem mass spectrometry for pharmacokinetic study in healthy subjects. Biomed Chromatogr. 2019 Oct 19;:e4708 Authors: Bharwad KD, Shah PA, Shrivastav PS, Sharma VS, Singhal P Abstract A rapid, simple and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method has been developed to quantify fenoprofen, a non-steroidal anti-inflammatory drug in human plasma for a pharmacokinetic study in healthy subjects. Due to high protein binding, protein precipitation followed by solid-phase extraction was employed for the extraction of fenoprofen and fenoprofen-d3 (used as internal standard) from 200 μL human plasma. Separation was performed on BEH C18 (50 × 2.1 mm, 1.7 μm) column using methanol-0.2 % acetic acid in water (75:25, v/v) under isocratic elution. Electrospray ionization was operated in the negative mode for sample ionization. Ion transitions used for quantification in the selected reaction monitoring mode were m/z 241/197 and m/z 244/200 for fenoprofen and fenoprofen-d3, respectively. Under the optimized conditions, fenoprofen showed excellent linearity in the concentration range of 0.02-20 μg/mL (r2 ≥ 0.9996), adequate sensitivity, favorable accuracy (96.4-103.7 %) and precision (% coefficient of variation ≤ 4.3) with negligible matrix effect. The validated method was successfully applied to a pharmacokinetic study of fenoprofe...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research