Simultaneous determination of proteins in microstructured optical fibers supported by chemometric tools.

Simultaneous determination of proteins in microstructured optical fibers supported by chemometric tools.
Anal Bioanal Chem. 2019 Oct 09;:
Authors: Burmistrova NA, Pidenko PS, Pidenko SA, Skibina YS, Monakhova YB
Abstract
A new perspective on the relevant problem-creating simple, rapid, and efficient protein sensors based on microstructured optical fibers using a simple homogeneous analysis format-was proposed. Commercially available long-period grating hollow core microstructured optical fibers (LPG HCMOF) were used to determine bovine serum albumin (BSA) and albumin from chicken eggs (OVA) in binary mixtures as well as immunoglobulin G (IgG) in the presence of BSA and OVA. LPG HCMOF transmission spectra allowed the detection of both BSA and OVA up to 10 mg/mL with LOD as low as 0.1 and 0.8 μg/mL, respectively. Partial least squares regression (PLS) was utilized for modeling of LPG HCMOF spectral data and quantitative analysis of BSA, OVA, total protein, and IgG in binary and ternary mixtures. Rather high coefficients of determination (R2) and low root mean square error for the calibration (RMSEC) (15%) and prediction (RMSEP) (20%) were obtained for all PLS models. The proposed approach was tested in the analysis of BSA in spiked horse blood hemolyzed (HBH). The results demonstrated the functionality of the proposed approach and offered the opportunity for the creation of a wide range of sensors for protein determination in comple...

Source: Analytical and Bioanalytical Chemistry - October 8, 2019 Category: Chemistry Authors: Burmistrova NA, Pidenko PS, Pidenko SA, Skibina YS, Monakhova YB Tags: Anal Bioanal Chem Source Type: research